多重耐药鸡致病性沙门氏菌I类整合子的检测研究

采用药敏纸片法（NCCLS 2004），对19株鸡致病性沙门氏菌进行了药敏试验，结果19株菌对丁胺卡那霉素、卡那霉素、庆大霉素、新霉素、链霉素、氨苄青霉素、强力霉素、氟哌酸、头孢三嗪、氯霉素、磺胺异恶唑、四环素、先锋V 13种药物的耐药率分别是：5.26%、63.16%、47.37%、36.84%、84.21%、84.2%、100%、57.9%、5.26%、52.6%、89.47%、100%、26.3%。19株菌多为多重耐药：其中耐5种、6种、7种、8种、9种、10种药物的分别为10.53%、 5.26%、15.79%、21.05%、15.79%、15.79%，多重耐药率84.21%（16/19）；根据I类整合子的结构设计了３对引物，对I类整合子的intI1、可变区、3’保守端进行扩增，检测鸡致病性沙门氏菌I类整合子,分析I类整合子与多重耐药的关系，扩增出了750～2000 bp的基因盒片段，检出率为73.68%（14/19），多重耐药菌的I类整合子检出率为87.5%（14/16），I类整合子PCR阳性菌株多重耐药率为100%（14/14）。研究结果表明，I类整合子与多重耐药之间有明显的相关性。

Study on Detection I Integron of Multidrug Resistace Pathogenic Salmonella from Chicken

Antimicrobial resistance test of 19 samonella isolates was done by disc dilution method(NCCLS 2003).According to the construction of I integron,The drug-resistance rate of amikacin,kanamycin,gentamicin,neomycin,streptomycin,ampicillin,doxycycline,norfloxacin,ceftriaxone,chloramphenicol,sulfafurazole,tetracycline,cefazolin,was 5.26%,63.16%,(47.37%),36.84%,84.21%,84.2%,100%,57.9%,5.26%,52.6%,89.47%,100%,26.3% respectively.Most of the isolates were multidrug resistance and the rate was 84.21%(16/19).Among the total of the multidrug-resistance isolates,the rate of resistance to five,six,seven,eight,nine and ten drugs was 10.53%,5.26%,15.79%,21.05%,15.79%,15.79%.PCR was employed to amplify I integron and analysis the relationship between I integron and multidrug resistance.3 pairs of primer was design to amplify I integron's intI1 gene,variable segment and 3'-conserved segment.As a result,we amplified several fragments with different length from 750 to 2000 bp. Among these 19 isolates,the detection rate of I integron was 73.68%(14/19);and among these 16 isolates which had been proven multidrug-resistant,the detection rate of I integron was 87.5%.The I integron positive isolates' rate of multidrug-resistance was 100%(14/14).The results shows I integron obviously has the relationship with multidrug resistance.