Prokaryotic Expression of Rice Ospgip1 Gene and Bioinformatic Analysis of Encoded Product |
| |
| Authors: | CHEN Xi-jun LIU Xiao-wei ZUO Si-min MA Yu-yin TONG Yun-hui PAN Xue-biao XU Jing-you |
| |
| Affiliation: | 1.Horticulture and Plant Protection College, Yangzhou University, Yangzhou 225009, China;2.Key Laboratory of Plant Functional Genomics of Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China |
| |
| Abstract: | Using the reference sequences of pgip genes in GenBank,a fragment of 930 bp covering the open reading frame(ORF) of rice Ospgip1(Oryza sativa polygalacturonase-inhibiting protein 1) was amplified.The prokaryotic expression product of the gene inhibited the growth of Rhizoctonia solani,the causal agent of rice sheath blight,and reduced its polygalacturonase activity.Bioinformatic analysis showed that OsPGIP1 is a hydrophobic protein with a molecular weight of 32.8 kDa and an isoelectric point(pI) of 7.26.The... |
| |
| Keywords: | Ospgip1 gene polygalacturonase-inhibiting protein prokaryotic expression bioinformatic analysis rice Rhizoctonia solani |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《水稻科学》浏览原始摘要信息 |
|
点击此处可从《水稻科学》下载全文 |
|
