高效液相色谱法测定玉米叶片中的酚酸类化合物

以植物中主要具有防御功能的8种酚酸作为对照，采用高效液相色谱法测得玉米叶片中主要含有香草酸、咖啡酸、丁香酸及香豆酸4种酚酸类化合物。选择Hypersil ODS C₁₈为色谱柱，乙腈-水体系为流动相，梯度洗脱，各酚酸实现有效分离。香草酸、咖啡酸、丁香酸和香豆酸4种酚酸类化合物在0～57.3 g/mL、0～95.1 g/mL、0～28.8 g/mL和0～47.7 g/mL浓度范围内具有良好的线性，相关系数大于0.999。加标回收率分别为101.2%～115.1%、88.7%～97.0%、106.5%～112.7%和96.8%～102.7%。在测定浓度范围内，该方法准确、可靠，可用于玉米等植物叶片中酚酸类化合物的分析测定。

Determination of Phenolic Acids on Corn Leaves by High Performance Liquid Chromatography

Used the main eight kinds of phenolic acids with plant-defensive function as a control,and a high performance liquid chromatography to detected the leaves of corn that containing vanillic acid, caffeic acid, syringic acid and coumaric acid. Four compounds were successfully separated on a Hypersil ODS C_(18) analytical column using a mobile phase of acetonitrile-water with gradient elution. Four phenolic acids exhibited good linear relationship in the concentrations ranging 0-57.3 g/mL, 0-95.1 g/mL, 0-28.8 g/mL and 0-47.7 g/mL, with the correlation coefficient higher than 0.999. Their recoveries were 101.2%-115.1%,88.7%-97.0%,106.5%-112.7% and 96.8%-102.7%, respectively. It could be seen that within the linear range, the method was accurate and reliable. Therefore, it could be used for determine the phenolic acids in the leaves of corn.