| 转苏云金杆菌毒蛋白基因水稻抗螟性的离体叶片测定法 |
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| 引用本文： | 叶恭银,胡萃,舒庆尧,崔海瑞,高明尉.转苏云金杆菌毒蛋白基因水稻抗螟性的离体叶片测定法[J].植物保护学报,2000,27(1):1-6. |
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| 作者姓名： | 叶恭银 胡萃 舒庆尧 崔海瑞 高明尉 |
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| 作者单位： | 浙江大学植物保护系,浙江大学植物保护系,浙江大学原子核农业科学研究所,浙江大学原子核农业科学研究所,浙江大学原子核农业科学研究所 杭州 310029,杭州 310029,杭州 310029,杭州 310029,杭州 310029 |
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| 基金项目： | 浙江省科委“九五”重大项目,美国洛克菲勒基金项目(RF97001#56) |
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| 摘 要： | 提出了转苏云金杆菌ｃｒｙｌＡｂ基因水稻抗螟性的快速离体叶片测定法,并用该方法评价了转ｃｒｙ１／Ａｂ基因水稻对二化螟、三化螟和大螟的抗性程度。结果表明,３种螟虫蚁螟取食纯合株系ＰＲ１６及分离株系ＰＲ１８、ＰＲ５７或ＰＲ５８中抗性植株中片后,食叶量与对照和分离株系中的非抗性植株相比极显著下降,接虫后第４ｄ的幼虫死亡率高达１００％。该方法评价转ｃｒｙ１／Ａｂ基因水稻对３种螟虫的抗性结果与活化成株测定法评
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| 关 键 词： | 苏云金杆菌 转基因水稻 螟虫 抗性 离体叶片测定 |
THE APPLICATION OF DETACHED-LEAF BIOASSAY FOR EVALUATING THE RESISTANCE OF Bt TRANSGENIC RICE TO STEM BORERS |
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| Ye Gongyin,Hu Cui,Shu Qingyao,Cui Hairui and Gao Mingwei.THE APPLICATION OF DETACHED-LEAF BIOASSAY FOR EVALUATING THE RESISTANCE OF Bt TRANSGENIC RICE TO STEM BORERS[J].Acta Phytophylacica Sinica,2000,27(1):1-6. |
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| Authors: | Ye Gongyin Hu Cui Shu Qingyao Cui Hairui and Gao Mingwei |
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| Institution: | Department of Plant Protection, Zhejiang University, Hangzhou 310029,Department of Plant Protection, Zhejiang University, Hangzhou 310029,Institute of Nuclear Agricultural Science, Zhejiang University, Hangzhou 310029,Institute of Nuclear Agricultural Science, Zhejiang University, Hangzhou 310029 and Institute of Nuclear Agricultural Science, Zhejiang University, Hangzhou 310029 |
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| Abstract: | The "detached-leaf bioassay", a rapid and easy method for determining the resistance of Bt (cry1Ab gene of Bacillus thuringiensis Berliner) transgenic rice to three stem borers, namely, striped stem borer Chilo suppressalis (Walker), yellow stem borer Scirpophaga incertulas (Walker) and purple stem borer Sesamia inferens (Walker), was developed and used to evaluate the resistance of different Bt transgenic rice lines to these stem borers. The results showed that after the newly hatched larvae of the three stem borers fed on the detached leaves of PR 16 (a homozygous Bt transgenic line) and resistant plants of PR18, PR57 and PR58 (the segregate Bt transgenic lines), their feeding amounts were significantly lower than those on the check variety (non-Bt-transgenic) and non-resistant plants of these segregate lines, all the former mortalities on the 4th day after inoculation were up to 100%. The test results of this method were quite coincident with those of the whole plant bioassay, both the two methods had indicated that the homozygous Bt transgenic line PR16 is highly resistant to the three stem borers. |
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| Keywords: | Bacillus thuringiensis transgenic rice rice stem borers resistance de-tached-leaf bioassay |
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