巨峰葡萄实生苗阶段发育过程中几种同工酶酶谱的变化

采用聚丙烯酰胺凝胶电泳,分析了三年生巨峰实生苗阶段发育过程中不同节位木质部的过氧化物酶、多酚氧化酶和超氧物歧化酶同工酶谱带。结果表明:不同节位木质部的过氧化物酶同工酶的一条谱带,从茎基部到首次出现卷须的部位其活性增强,随后便消失。在休眠期,过氧化物酶同工酶不存在阶段发育的酶谱差异性;在生长期则随着葡萄蔓阶段发育程度的提高酶带数增多,活性增强。超氧物歧化酶同工酶,不论是休眠植株还是活跃生长的植株,其酶谱保持不变,但休眠期酶带多、活性高。过氧化物酶同工酶与多酚氧化酶同工酶酶谱类型一致。

STUDY ON THE POX,PPO AND SOD IN SEEDLINGS OF 'KYOHO'GRAPE VARIETY IN THE COURSE OF THEIR PHASE DEVELOPMENT

The activity and isozymic patterns of the peroxidase (POX), polyphenol oxidase (PPO) and superoxide disrnutase (SOD) extracted from stem xylem in the nodes which were not the same degree of development of thre e-yead-old 'Kyoho' grape seedlings were analyzed by using the discontinuous system of poly acrylamid gel electrophorsis. It was shoown that a specific band on the electrophoretogram of POX from stem segments increased in activity from the stem base up to the first-tendril position and then disappeared. During the rest period, no difference in enzyme patterns between development stages was found whereas during the growing season number and activity of POX increased steadily as the development of grapevine advanced. The isozyme pattern of SOD, however, remained unchanged with the advancement of the development process, regardless of whether the tissue was sampled from a dormant oractively growing plant More bands and higher actlvity of this isozyme were found during the dormant period. The zymograms of PX and PPO were proved to be identical.