| 烟草根黑腐病菌的PCR分子检测 |
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| 引用本文： | 赵永强,张成玲,张薇,李璐宁,张广民.烟草根黑腐病菌的PCR分子检测[J].植物病理学报,2009,39(1):23-29. |
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| 作者姓名： | 赵永强 张成玲 张薇 李璐宁 张广民 |
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| 作者单位： | 山东农业大学植物保护学院, 泰国 271018 |
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| 基金项目： | 山东省烟草专卖局科技项目 |
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| 摘 要： | 根据烟草根黑腐病菌(Thielaviopsis basicola)与其它烟草病原真菌核糖体基因转录间隔区(internal transcribed spa-cer,ITS)序列间的差异,设计了一对特异性引物TB-5/TB-3,用于T. basicola的分子检测。利用该对引物对包括T. basicola在内的13个烟草病原菌菌株的基因组DNA进行PCR扩增,结果表明:只有T. basicola能扩增到一条400bp左右的特异性条带,其它菌株及阴性对照均无扩增产物。对烟草组织和土壤的检测结果也表明,该对引物能特异性的检测到T. basicola基因组DNA的存在。该引物对T. basicola基因组DNA检测的灵敏度为100fg/μL。
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| 关 键 词： | 烟草 根黑腐病菌 ITS PCR 分子检测 |
Molecular detection of Thielaviopsis basicola by PCR assay |
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| ZHAO Yong-qiang,ZHANG Cheng-ling,ZHANG Wei,LI Lu-ning,ZHANG Guang-min.Molecular detection of Thielaviopsis basicola by PCR assay[J].Acta Phytopathologica Sinica,2009,39(1):23-29. |
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| Authors: | ZHAO Yong-qiang ZHANG Cheng-ling ZHANG Wei LI Lu-ning ZHANG Guang-min |
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| Institution: | College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, China |
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| Abstract: | Based on the differences in internal transcribed spacer (ITS) sequences of T. basicola and other tobacco fungal pathogens, a pair of species-specific primers TB-5/TB-3 was designed for T. basicola detection. After screening 10 isolates of T. basicola and other tobacco fungal pathogens, TB-5/TB-3 primers amplified only a single product of about 400 bp from T. basicola. And T. basicola could be specifically dectec-ted by PCR assay with TB-5/TB-3 from tobacco tissues infected naturally and soil samples. The sensitivity of detection was 100 fg genomic DNA per 25 μL PCR reaction volume. The PCR-based methods developed here could simplify both plant disease diagnosis and pathogen monitoring, as well as guiding plant disease management. |
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| Keywords: | ITS PCR |
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