| RP-HPLC法测定大叶香茶菜中冬凌草甲素含量 |
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| 引用本文： | 王永学,王中全,陈正跃,邱培勇,张积霞. RP-HPLC法测定大叶香茶菜中冬凌草甲素含量[J]. 安徽农业科学, 2008, 36(22) |
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| 作者姓名： | 王永学 王中全 陈正跃 邱培勇 张积霞 |
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| 作者单位： | 1. 新乡医学院药物研究室,河南新乡,453003
2. 河南省新乡市公安局,河南新乡,453003 |
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| 基金项目： | 河南省科技厅科技攻关项目 |
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| 摘 要： | [目的]应用RP-HPLC法测定大叶香茶菜中冬凌草甲素的含量。[方法]RP-HPLC法色谱条件:色谱柱为Varian Microsorb-MV 100-5C18柱,流动相为甲醇∶水(1∶1),流速1.0 ml/min,检测波长λ=238 nm,柱温30℃。[结果]冬凌草甲素加样回收率为98.6%,RSD为2.04%(n=5);大叶香茶菜叶中冬凌草甲素的含量为4.79 g/kg。[结论]该法简便可靠,适用于大叶香茶菜中冬凌草甲素含量测定。
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| 关 键 词： | 大叶香茶菜 冬凌草甲素 RP-HPLC法 |
Quantitative Determination of Oridonin in Isodon macrophyllus by RP-HPLC |
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| WANG Yong-xue et al. Quantitative Determination of Oridonin in Isodon macrophyllus by RP-HPLC[J]. Journal of Anhui Agricultural Sciences, 2008, 36(22) |
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| Authors: | WANG Yong-xue et al |
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| Abstract: | [Objective] The research aimed to determine the content of oridonin in Isodon macrophyllus by RP-HPLC method.[Method] The chromatographic conditions of RP-HPLC method were that the chromatographic column was analytical C18 column, the mobile phase was methanol-water(1∶1),the flow rate was 1.0 ml/min,the column temperature was 30 ℃,the detection wavelength was 238 nm.[Result] The average recovery was 98.6% with RSD 2.04%(n=5).The content of oridonin in Isodon macrophyllus was 4.79 g/kg.[Conclusion] This method was proved to be simple and reliable.It could be used for the oridonin content detemination in Isodon macrophyllus. |
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| Keywords: | Isodon macrophyllus Oridonin HPLC |
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