| ERIC-PCR技术特点及其应用 |
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| 引用本文： | 李犹平,倪学勤.ERIC-PCR技术特点及其应用[J].安徽农业科学,2007,35(18):5358-5360. |
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| 作者姓名： | 李犹平 倪学勤 |
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| 作者单位： | 四川农业大学动物科技学院,四川雅安,625014;四川农业大学动物科技学院,四川雅安,625014 |
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| 摘 要： | ERIC序列是近年来发现的存在于原核生物基因组中一类短的重复序列。该序列在染色体上的分布和拷贝数具有种间的特异性,根据序列中心高度保守的44 bp的ERIC核心序列设计反向引物,可扩增出反映细菌基因组结构特征的谱带。由于ERIC-PCR快速简便,图谱重复性好,能用于细菌分类鉴定、环境监测、污染治理以及人和动物肠道菌群结构研究等方面。
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| 关 键 词： | ERIC IRU ERIC-PCR 特点 应用 |
| 文章编号： | 0517-6611（2007）18-05358-03 |
| 修稿时间： | 2007-03-23 |
Characteristic and Application of the Enterobacterial Repetitive Intergenic Consensus-PCR |
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| LI You-ping et al.Characteristic and Application of the Enterobacterial Repetitive Intergenic Consensus-PCR[J].Journal of Anhui Agricultural Sciences,2007,35(18):5358-5360. |
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| Authors: | LI You-ping |
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| Institution: | College of Animal Science and Technology ,Sichuan Agricultural University,Yaan,Sichuan 625014 |
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| Abstract: | Enterobacterial Repetitive Intergenic Consensus(ERIC),a kind of short duplication sequences,is found to exist in the genome of prokaryotic organism.Distribution and copy of the sequence in chromosome is interspecific specificity.According to the highly conservative core sequence,44bp,reverse primer is designed.ERIC-PCR can generate unique fingerprints to reflect the structural feature of bacterial strains,and can identification species.ERIC-PCR technique can be used in the identification of bacterium,environmental monitoring,investigation intestinal flora of human beings and animals,which is rapid,simple and repetitive. |
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| Keywords: | ERIC IRU ERIC-PCR Characteristic Application |
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