| 离子色谱-积分脉冲安培检测法测定榴莲中的17种氨基酸 |
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| 引用本文： | 吴伶俐,刘娜,徐鹏,牛云辉,卫学青,李卫华,黄晓书.离子色谱-积分脉冲安培检测法测定榴莲中的17种氨基酸[J].安徽农业科学,2009,37(8):3357-3359. |
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| 作者姓名： | 吴伶俐 刘娜 徐鹏 牛云辉 卫学青 李卫华 黄晓书 |
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| 作者单位： | 河南广播电视大学农医学院,河南郑州,450008;河南农业大学生物技术重点实验室,河南郑州,450002;戴安中国有限公司,北京,100029 |
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| 基金项目： | 郑州市技术研究与开发经费支持项目(074SCCG36112-3) |
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| 摘 要： | 目的]了解榴莲各部分的食用和药用价值，以便为其开发和综合利用提供依据。方法]采用AminoPaePA—10氨基酸分析柱，用积分脉冲安培离子色谱法测定榴莲中17种氨基酸及其含量。结果]各组分的线性关系良好，相关系数在0．9907～0．9998，加标回收率在80．0％～112．9％，检出限在0．0028～0．0136μmol／L之间。pH=12～13时，氨基酸的洗脱顺序大体是：碱性氨基酸、中性氨基酸、酸性氨基酸。流动相中Ac^-与阴离子交换树脂的亲和力要大于OH^-。各氨基酸在0．05～25．0nmol／L范围内线性关系良好。精密度检测的RSD在1．2％-2．7％。重复性检测的RSD在2．3％-8．4％。加标回收试验的平均回收率在80．O％～112．9％。结论]该法具有灵敏度高、精密度好、分离速度快和样品不需要衍生处理的优点。
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| 关 键 词： | 离子色谱法 榴莲 氨基酸 |
Determination of 17 Amino Acids in Durian by Ion Chromatography-Integrated Pulsed Amperometric Detection |
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| WU Ling-li et al.Determination of 17 Amino Acids in Durian by Ion Chromatography-Integrated Pulsed Amperometric Detection[J].Journal of Anhui Agricultural Sciences,2009,37(8):3357-3359. |
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| Authors: | WU Ling-li |
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| Institution: | WU Ling-li et al(College of Agriculture , Medicine,Henan Radio & Television University,Zhengzhou,Henan 450008) |
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| Abstract: | Objective] The aim was to learn about the edible and medicine value of all the parts of durian so as to supply basis for its expolitation and comprehensive utilization.Method] AminoPac PA-10 amino acid analysis column was used and 17 amino aids in durian and their contents were detected and determined by ion chromatography-integrated pulsed amperometric method.Result] The linear relationships among components were good.Their correlation coefficients were between 0.990 7 and 0.999 8,their recoveries of st... |
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| Keywords: | Ion chromatography Durian Amino acid |
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