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川芎茵核病的症状及病原菌鉴定
引用本文:罗玲,黄云,王靖,何苗,万英.川芎茵核病的症状及病原菌鉴定[J].植物病理学报,2009,39(5):532-535.
作者姓名:罗玲  黄云  王靖  何苗  万英
作者单位:四川农业大学植物病理学系, 雅安 625014
基金项目:四川省科技厅攻关招标项目 
摘    要: Symptoms of sclerotinia stem rot of Ligusticum chuanxiong were observed and the causal agent of the disease was identified. The results showed that the symptoms of this disease began with the formation of dark brown hygrophanous splotch on the basal leaves, followed by the appearance of dark brown circular spot on the basal stem. Finally, the whole plant stoped growing and died. White mycelium and/or sclerotia were formed under moisturized conditions. On PDA medium, the pathogen of this disease formed brown sclerotia with various shapes and 2-5 mm in diameter. The diameter of apothecia was 0.3-0.8 cm and the length of stipes was 2-8 cm. Asci were columniform in shape and (100-135)×9μm in size, each containing 8 ascospores sized by (6-12) μm×(3-7)μm. No asexual spore formation was observed. Based on the morphological characteristics and the sequence of ribosomal DNAITS, the pathogen was identified as Sclerotinia sclerotiorum (Lib.) de Bary.


Symptoms of sclerotinia stem rot on Ligusticum chuanxiong and Identification of its pathogen
LUG Ling,HUANG Yun,WANG Jing,HE Miao,WAN Ying.Symptoms of sclerotinia stem rot on Ligusticum chuanxiong and Identification of its pathogen[J].Acta Phytopathologica Sinica,2009,39(5):532-535.
Authors:LUG Ling  HUANG Yun  WANG Jing  HE Miao  WAN Ying
Institution:Department of Plant Pathology, Sichuan Agricultural University, Ya'an 625014, China
Abstract:Symptoms of sclerotinia stem rot of Ligusticum chuanxiong were observed and the causal agent of the disease was identified. The results showed that the symptoms of this disease began with the formation of dark brown hygrophanous splotch on the basal leaves, followed by the appearance of dark brown circular spot on the basal stem. Finally, the whole plant stoped growing and died. White mycelium and/or sclerotia were formed under moisturized conditions. On PDA medium, the pathogen of this disease formed brown sclerotia with various shapes and 2-5 mm in diameter . The diameter of apothecia was 0.3 -0.8 cm and the length of stipes was 2 -8 cm. Asci were columniform in shape and (100 - 135) ×9 μm in size, each containing 8 ascospores sized by (6-12) μm × (3 -7) μm. No asexual spore formation was observed. Based on the morphological characteristics and the sequence of ribosomal DNA-ITS, the pathogen was identified as Sclero-tinia sclerotiorum (Lib. ) de Bary.
Keywords:sclerotinia stem rot  Ligusticum chuanxiong  symptoms  pathogen  identification
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