| 高固氮基因工程大豆根瘤菌的研制及其增氮效应 |
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| 引用本文： | 刘涛,周俊初,柏学亮,沈辉,张学贤,马庆生. 高固氮基因工程大豆根瘤菌的研制及其增氮效应[J]. 广西农业生物科学, 1996, 0(1) |
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| 作者姓名： | 刘涛 周俊初 柏学亮 沈辉 张学贤 马庆生 |
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| 作者单位： | 广西农业大学实验中心分子遗传学研究室，华中农业大学生命科学院 |
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| 基金项目： | 国家863高技术研究发展计划资助 |
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| 摘 要： | 通过构建快生型大豆根瘤菌Ｂ５２的基因文库和三亲本杂交，将增效因子ＤＮＡ片段导入优良的慢生型大豆根瘤菌２２－１０中，获得携带来自快生菌增效因子ＤＮＡ片段的工程菌株ＨＮ３２，经盆栽和小区试验，证明基因工程菌株ＨＮ３２比出发菌株２２－１０平均增产６％，比对照平均增产１３．２％～１６．９％，相当于每公顷施７５～１５０ｋｇ尿素．１９９２～１９９５年，在广西推广应用基因工程大豆根瘤菌ＨＮ３２２．１６万ｈｍ２，每公顷平均增产１９％，投入产出比１：３０。增加经济效益１４０９．８万元。
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| 关 键 词： | 基因文库，基因工程；大豆根瘤菌，增氮效应 |
Study on A High Nitrogen Fixation Engineering Strain of Bradyrhizobium japonicum and Its Effect in Nitrogen Fixation Enhancement |
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| Liu Tao, Zhou Junchu, Bai Xueliang, Sheng Hui, Zhang Xuexian'''' Ma Qingsheng. Study on A High Nitrogen Fixation Engineering Strain of Bradyrhizobium japonicum and Its Effect in Nitrogen Fixation Enhancement[J]. Journal of Guangxi Agricultural and Biological Science, 1996, 0(1) |
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| Authors: | Liu Tao Zhou Junchu Bai Xueliang Sheng Hui Zhang Xuexian'''' Ma Qingsheng |
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| Abstract: | A synergistic DNA fragment from a Rhizobium fredii strain B52 was cloned and transferred to Bradyrhizobium japonicum strain 22-10 by triparental mating, and an engineering strain HN32 which contains the synergistic DNA fragement of the strain B52 has been obtained. By pot plant experiment and field test, it was shown that the engineering strain HN32's productivity reached 106% of the original strain 22-10 and 113. 2% to 116. 9% of the control, and its nitrogen fixation capacity equaled to the application of 75 to 150 kg urea per hectare. From 1992 to 1995, the engineering strain HN32 has been used over 21 600hectares in Guangxi and the average yield was increased up to 19%. The ratio of input/output is 1: 30. |
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| Keywords: | gene bank gene engineering Bradyrhizobium japonicum nitrogen fixation enhancement |
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