赤霉素与小麦生理型雄性不育的关系

为探究赤霉素与小麦生理型雄性不育的关系,以新型小麦化学杀雄剂SQ-1作为诱导剂,西农1376为材料,构建了西农1376不育和可育生理系,通过扫描电镜、荧光定量PCR、气相质谱等技术,研究了小孢子花粉形态、TaGAMYB和CYP709C1基因表达、硬脂酸和赤霉素含量和花粉育性。结果表明,化学杀雄剂SQ-1诱导的生理型雄性不育系花粉粒畸形,TaGAMYB和CYP709C1基因表达量显著下降,硬脂酸含量显著上升,赤霉素含量显著低于可育系。赤霉素对雄性不育系的育性有一定逆转效应。

Relationship between Gibberellin and Chemical-Induced Male Sterility in Wheat

The study was conducted in order to reveal the relationship between gibberellin and chemicalinduced male sterility in wheat(Triticum aestivum L).Wheat variety Xinong 1376 was explored to develop physiological male sterile line through spraying SQ-1(a new chemical agent) on wheat plants at Feeke's 8.5 stage,with the plants sprayed with same volume water as the control.Pollen morphology,TaGAMYB and CYP709C1 expression,octadecanoic acid content,and gibberellin content were analyzed via techniques such as scanning electron microscope,fluorescence quantitative PCR,gas chromatography mass spectrum at mononuclear stage.The results indicated that pollen deformity of chemical-induced male sterility generated,and expression patterns of TaGAMYB and CYP709C1 were downregulated significantly,and instead,octadecanoic acid content rise sharply.Meanwhile,gibberellin content was significantly lower than that of fertile line.Gibberellin have a reverse effect on sterility of male sterile lines.