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棉花黄萎病生防内生细菌Jaas cd的鉴定及田间防效
引用本文:林玲,金中时,马长文,孙飞,王凤良,龚伟荣,顾本康,周益军.棉花黄萎病生防内生细菌Jaas cd的鉴定及田间防效[J].江苏农业学报,2010,26(1).
作者姓名:林玲  金中时  马长文  孙飞  王凤良  龚伟荣  顾本康  周益军
作者单位:1. 江苏省农业科学院植物保护研究所,江苏,南京,210014
2. 江苏省大丰市植保植检站,江苏,大丰,224100
3. 江苏省植物保护站,江苏,南京,210013
基金项目:国家“863”计划项目(2006AA10A211);;“十一五”国家科技支撑计划项目(2006BAD08A07-02);;农业部公益性行业专项(nyhyzx07-052);;农业部转基因专项(2009ZX08005-003B);;江苏省农业科学院基金项目(6110826);;江苏省农业科学院学科带头人优秀后备人才项目(6510816)
摘    要:对棉花黄萎病生防内生细菌Jaas cd(原名73a)进行了菌种鉴定和田间应用方式的改进。抑菌谱测定结果表明,该菌株对12种病原真菌均有较强的抑制作用。通过形态观察、生理生化鉴定、Biolog鉴定以及16S rD-NA序列比对,证明该菌株为多黏类芽孢杆菌(Paenibacillus polymyxa)。菌株Jaas cd的16S rDNA序列与多黏类芽孢杆菌(AM062684)的相似性为99.7%,GenBank登录号为AY942618。田间大区示范试验结果表明,用菌株Jaascd的发酵液在棉苗移栽前喷施棉苗和移栽时灌根2种方式处理,都能有效防治棉花黄萎病和提高棉花产量,但移栽前喷施棉苗操作更加简便易行。

关 键 词:内生细菌  多黏类芽孢杆菌  棉花黄萎病  

Identification and Field Control Efficacy of Endophytic Bacterial Strain Jaas cd against Cotton Verticillium Wilt
LIN Ling,JIN Zhong-shi,MA Chang-wen,SUN Fei,Wang Feng-liang,GONG Wei-rong,GU Ben-kang,ZHOU Yi-jun.Identification and Field Control Efficacy of Endophytic Bacterial Strain Jaas cd against Cotton Verticillium Wilt[J].Jiangsu Journal of Agricultural Sciences,2010,26(1).
Authors:LIN Ling  JIN Zhong-shi  MA Chang-wen  SUN Fei  Wang Feng-liang  GONG Wei-rong  GU Ben-kang  ZHOU Yi-jun
Institution:1.Institute of Plant Protection/a>;Jiangsu Academy of Agricultural Sciences/a>;Nanjing 210014/a>;China/a>;2.Dafeng County Station of Plant Protection and Quarantine/a>;Dafeng 224100/a>;3.Jiangsu Plant Protection Station/a>;Nanjing 210013/a>;China
Abstract:An endophytic bacteria against cotton Verticillium wilt,strain Jaas cd(original name 73a),was identified and its application methods in the field were improved.The strain showed the strong antifungal activity against 12 fungal pathogens including Verticillium dahliae Kleb,Fusarium oxysporum f.sp.vasinfectum,Fusarium moniliforme and Rhizoctonia solani.According to the morphological and physiological characteristics,Biolog identification and phylogenetic analysis,strain Jaas cd was identified as Paenibacillus...
Keywords:endophytic bacterium  Paenibacillus polymyxa  cotton Verticillium wilt  
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