| 牛BMP3基因cDNA克隆及组织表达谱分析 |
| |
| 引用本文： | 张小辉,高雪,任红艳,陈金宝,许尚忠.牛BMP3基因cDNA克隆及组织表达谱分析[J].河南农业大学学报,2009,43(2). |
| |
| 作者姓名： | 张小辉 高雪 任红艳 陈金宝 许尚忠 |
| |
| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所,北京,100094;河南科技大学动物科技学院,河南,洛阳,471003
2. 中国农业科学院北京畜牧兽医研究所,北京,100094 |
| |
| 摘 要： | 用RT-PCR的方法克隆了鲁西黄牛BMP3基因1 555 bp的eDNA序列,该序列包含1个1 428 bp的完整开放阅读框,编码476个氨基酸残基;通过对推导的牛BMP3蛋白进行生物信息学分析发现,牛BMP3蛋白的分子量为53 506.27 D,等电点为9.42,该蛋白前22个氨基酸残基为信号肽序列,而亚细胞定位分析发现,该成熟蛋白可能位于细胞外.通过与最新公布的牛基因组比对,发现牛BMP3基因位于牛基因组第6号染色体上,由3个外显子和2个内含子组成,牛BMP3基因cDNA序列与人、小鼠、大鼠和鸡BMP3基因cDNA序列的相似性分别达到84%,81%,81%,79%,是进化上保守的基因.在牛卵巢、肝、肌肉、小肠、脂肪、子宫、肾脏、心肌、肺、胰腺等组织中都检测到BMP3基因表达,表明牛BMP3基因是一个功能重要、进化保守的基因,具有广泛的组织表达谱.
|
| 关 键 词： | 牛 cDNA克隆 组织表达 |
Cloning and tissues expression analysis of cDNA of the cattle BMP3 gene |
| |
| ZHANG Xiao-hui,GAO Xue,REN Hong-yan,CHEN Jin-bao,XU Shang-zhong.Cloning and tissues expression analysis of cDNA of the cattle BMP3 gene[J].Journal of Henan Agricultural University,2009,43(2). |
| |
| Authors: | ZHANG Xiao-hui GAO Xue REN Hong-yan CHEN Jin-bao XU Shang-zhong |
| |
| Institution: | 1.Institution of Animal Huskandry and Veterinary Science;Chinese Academy of Agricultural Sciences;Beijing 100094;China;2.College of Animal Science and Technology;Henan University of Science and Technology;Luoyang 471003;China |
| |
| Abstract: | The Luxi cattle BMP3 gene cDNA was cloned by RT-PCR and a 1 555 bp cDNA sequence was obtained,including a 1 428 bp whole ORF region and encoding 476 animo acid residuals.The molecular weight of predicted cattle BMP3 protein was 53 506.27 D and the isoelectric point was 9.42.The first 22 amino acids in N-terminal was signal peptide.The maturation protein was predicted to exist out of cells by subcellular localization analysis.The cattle BMP3 gene consists of three exons and two introns,which were located in ... |
| |
| Keywords: | BMP3 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
