'白核'龙眼种子败育不同时期差异蛋白分析

以'白核'龙眼种子为试验材料,比较两种蛋白质的提取方法,确定了酚抽法提取龙眼种子蛋白.应用蛋白质组学研究技术,分析了龙眼种子败育4个不同时期的蛋白质组变化,共发现21个差异蛋白,其中9个上调表达,12个下调表达.通过MALDI/TOF/TOF-MS/MS质谱分析和蛋白质数据库检索,共鉴定出12个差异蛋白,分别为胞质苹果酸脱氢酶、丙酮酸脱氢酶、磷酸甘油酸变位酶、RuBisCO大亚基结合蛋白α、β亚基、17 kD HSP、抗坏血酸过氧化物酶、胞质抗坏血酸过氧化物酶、半胱氨酸蛋白酶、Dessication-related protein以及两个未知蛋白.这些鉴定出的差异蛋白质与能量和物质代谢、分子伴侣功能、自由基清除和抗氧化作用、细胞凋亡等生理过程密切相关,可能参与了龙眼种子败育过程.

Analysis of Differentially Expressed Proteins During the Different Stages of Longan Seed Abortion

By comparing with two protein extraction methods,ensured the optimal method for longan seed Droteins extraction.Proteomic approaches were used to analyze the changes of differentially expressedproteins during four different stages of longan seed abortion.Twenty-one differentially expressed proteins were detected on 2-DE gels,9 were up-regulated and 12 were down-regulated.Among these proteins,12 proteins were identified bv MALDI/TOF/TOF-MS/MS and protein database searching.They were cytosolic malate dehvdrogenase,Dihydrolipoamide adetyhransferase,2,3-bisphosphoglycerate-independent phosphoglycerate mutase,RuBisCO large subunit-binding protein subunit beta chloroplast precursor,RuBisCO large subunitbinding protein subunit beta chloroplast precursor,17 kD heat shock protein,ascorbate peroxidase,cytosolic ascorbate peroxidase,cysteine protease,dessication-related protein,and two unknown protein.These proteins were involved in energy and metabolism,chaperon,antioxidation,apoptosis,which might relate to seed abortion of longan.