| 蓝太阳鱼生长激素全长cDNA的克隆与序列分析 |
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| 引用本文： | 曹运长. 蓝太阳鱼生长激素全长cDNA的克隆与序列分析[J]. 水产学报, 2004, 28(5): 589-593 |
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| 作者姓名： | 曹运长 |
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| 作者单位： | 中山大学水生经济动物研究所暨广东省水生经济动物繁殖重点实验室,广东,广州,510275;广东省番禺国家级罗非鱼良种场,广东,广州,511453 |
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| 基金项目： | 国家自然科学基金专项基金项目 (3 9970 586)，广东省自然科学基金项目 (2 0 0 2 3 0 0 2 ) |
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| 摘 要： | The full length cDNA encoding growth hormone of a freshwater fish, Lepomis cyanellus, (LcGH) was cloned from pituitary RNA with RT-PCR, 3‘ and 5‘ RACE (rapid amplification of cDNA ends). The LcGH cDNA (Genbank No. AY530822), about 989nt (nucleotide) long, consisted of a open reading frame with 615nt long, 5‘and 3‘untranslated regions with 93nt and 224nt long respectively, and a 57nt poly (A) tail. The DNA sequence analysis showed that there are typical Kozak sequence and polyadenylation signal. The pregrowth hormone peptide of 204aa deduced from LcGH cDNA included a putative signal peptide (17aa) locating in its Nterminal. There exist a Asn-Cys-Thr glycosylation site at amino acid 201, and 4 cysteine residues (No. 69, 177, 194, 202) that are essential to construct two S-S bonds in this pregrowth hormone peptide. Homological comparision among LcGH and other species growth hormones showed that There is high homology (more than 85%) between growth hormone of Lepomis cyanellus and that of most perciformes fish, but low homology (less than 70%) in comparison with other species such as Siluriformes and Cypriniformes fish.
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| 关 键 词： | 蓝太阳鱼 生长激素 逆转录-聚合酶链式反应 cDNA末端快速扩增 |
| 文章编号： | 1000-0615(2004)05-0589-05 |
| 收稿时间： | 2014-03-14 |
| 修稿时间： | 2003-07-14 |
Cloning and sequencing of full length growth hormone cDNA from Lepomis cyanellus |
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| CAO Yun-chang. Cloning and sequencing of full length growth hormone cDNA from Lepomis cyanellus[J]. Journal of Fisheries of China, 2004, 28(5): 589-593 |
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| Authors: | CAO Yun-chang |
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| Affiliation: | CAO Yun-chang~1,LI Wen-sheng~1,YE Wei~2,LIN Hao-ran~1for Aquatic Economical Animal,Zhongshan University,Guangzhou510275,China,2.National Tilapia Breeding Farm of Guangdong Province,Guangzhou511453,China) |
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| Abstract: | The full length cDNA encoding growth hormone of a freshwater f ish, Lepomis cyanellus, (LcGH) was cloned from pituitary RNA with RT-PCR, 3..and 5.. RACE ( rapid amplif ication of cDNA ends) . The LcGH cDNA ( Genbank No. AY530822) , about 989nt ( nucleotide) long, consisted of a open reading frame with 615nt long, 5..and 3..untranslated regions with 93nt and 224nt long respectively, and a 57nt poly ( A) tail. The DNA sequence analysis showed that there are typical Kozak sequence and polyadenylation signal. The pregrowth hormone peptide of 204aa deduced from LcGH cDNA included a putative signal peptide ( 17aa) locating in its Nterminal. There exist a Asn-Cys-Thr glycosylation site at amino acid 201, and 4 cysteine residues ( No. 69, 177, 194, 202) that are essential to construct two S-S bonds in this pregrowth hormone peptide. Homological comparision among LcGH and other species growth hormones showed that There is high homology ( more than 85% ) between growth hormone of Lepomis cyanellus and that of most perciformes fish, but low homology ( less than 70%) in comparison with other species such as Siluriformes and Cypriniformes f ish. |
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| Keywords: | Lepomis cyanellus growth hormone RT-PCR RACE |
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