Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA) |
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| Authors: | S Winston S Fiscus L Hesterberg T Matsushita M Mildbrand J Porter Y Teramoto |
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| Institution: | Allelix Inc., Mississauga, Ontario. |
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| Abstract: | The development of three separate rapid ELISAs for detecting antibodies in host serum to three different viruses is described. These include: 1. A direct antigen assay using enzyme labelled anti-canine Ig for detecting antibodies to canine parvovirus, 2. A competitive ELISA using a feline infectious peritonitis virus-specific monoclonal antibody labelled with enzyme, and 3. A competitive ELISA using an equine infectious anemia virus-specific monoclonal antibody and enzyme labelled antigen, p. 26. The utility and benefits of each of the three approaches is emphasized. |
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