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影响梨叶片高频不定梢再生因素的研究
引用本文:孙清荣,刘庆忠,赵红军,刘 鹏. 影响梨叶片高频不定梢再生因素的研究[J]. 中国农学通报, 2004, 20(4): 99-99
作者姓名:孙清荣  刘庆忠  赵红军  刘 鹏
作者单位:山东省果树研究所,泰安,271000
基金项目:山东省优秀中青年科学家科研奖励基金项目(01DS48),山东农科院青年基金项目
摘    要:本文研究了基因型、基本培养基、植物生长物质、叶片接种方法及光、暗培养条件等因素对梨叶片不定梢再生的影响。基因型对叶片不定梢再生影响最大,安久和丰水的再生率最高,可达100%,其次为鸭梨,为80%,黄金梨的再生率最低,仅为27.3%。不同基因型其获得最高不定梢再生率所需附加的植物生长物质不同。TDZ比BA更有利于鸭梨叶片的高频不定梢再生,而BA更有利于西洋梨和砂梨叶片的不定梢再生。暗培养3周后再转光下培养比直接光培养有利于不定梢的再生,叶片接种方法对再生率无明显影响。

关 键 词:  叶片  不定梢再生

The Study of Factors of Effecting on High Frequency Shoot RegenerationFrom in vitro Leaves in Pear
Sun QingRong,Liu QingZhong,Zhao HongJun,Liu Peng. The Study of Factors of Effecting on High Frequency Shoot RegenerationFrom in vitro Leaves in Pear[J]. Chinese Agricultural Science Bulletin, 2004, 20(4): 99-99
Authors:Sun QingRong  Liu QingZhong  Zhao HongJun  Liu Peng
Affiliation:(Shandong Institute of Pomology, Taian, Shandong271000)
Abstract:The factors affecting on shoot regeneration from leaves in pear were studied. These factors included genotype, basic media, plant growth regulators, ways of leaf inoculation and culture condition of dark and light. Genotype is the most important factor of affecting shoots regeneration from leaves. The highest rate of shoots regeneration were reached from D'Anjou and Hosui, both reached 100%,the lowest is Yellow Gold pear, only 27.3%. Plant growth regulators needed for different genotypes reaching the highest regeneration rate were different. TDZ is more effective than BA for inducing shoots regeneration from Duckpear, and BA is more effective than TDZ for inducing shoots regeneration from Pyrus communis L. and P. pyrifolia Nak. Shoots regeneration is improved by culturing 3 weeks under dark. The ways of leaf inoculation on media had less effect on shoot regeneration.
Keywords:Pear  Leaves in vitro  Shoot regeneration  
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