毛葡萄穗轴褐腐病病原菌鉴定及其生物学特性

罗城仫佬族自治县是广西毛葡萄主产区。穗轴褐腐病是近年为害毛葡萄花穗穗轴的主要病害。作者根据病原菌形态、致病性,并利用分子鉴定方法,将该病的致病菌鉴定为可可毛色二孢(Lasiodiplodia theobromae)。该病菌生长最适温度为28~30℃;可生长pH值为3~11.5,其中,pH 3~4利于病原菌产生分生孢子;能有效利用蔗糖、葡萄糖、可溶性淀粉等14种供试碳源,但以葡萄糖最好;能利用DL-丙氨酸、硝酸钠、DL-甲硫氨酸等10种供试氮源,但以DL-丙氨酸最适。

Identification and biological characteristics of the pathogen causing brown rachis rot of Vitis heyneana

Luocheng Moulao Autonomous County is the main production area of Vitis heyneana Roem. et Schult in Guangxi, China. Brown rachis rot is the major disease at florescence stage, and becoming a constraint for fruit production of V. heyneana in recent years. The pathogen was identified as Lasiodiplodia theobromae according to the morphology, pathogenicity and sequence analysis of the internal transcribed spacer region of the ribosomal DNA (ITS). The optimum temperature for mycelial growth was 28-30℃. The pathogen could grow under pH 3-11.5 and pH 3-4 was the suitable range for sporulation. Sucrose and sodium nitrate could be used as better carbon and nitrogen sources for growth of L. theobromae, respectively.