| 氯霉素的直接竞争时间分辨荧光免疫检测法 |
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| 引用本文： | 张艺,黄飚,王柯,张珏,朱岚,马智鸿,周彬. 氯霉素的直接竞争时间分辨荧光免疫检测法[J]. 安徽农业科学, 2009, 37(27): 12912-12914 |
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| 作者姓名： | 张艺 黄飚 王柯 张珏 朱岚 马智鸿 周彬 |
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| 作者单位： | 江苏省原子医学研究所,江苏无锡,214063;江苏省原子医学研究所,江苏无锡,214063;江苏省原子医学研究所,江苏无锡,214063;江苏省原子医学研究所,江苏无锡,214063;江苏省原子医学研究所,江苏无锡,214063;江苏省原子医学研究所,江苏无锡,214063;江苏省原子医学研究所,江苏无锡,214063 |
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| 摘 要： | [目的]采用时间分辨荧光技术建立氯霉素（CAP）直接竞争免疫检测法（CAPTRFIA）。[方法]以羊抗鼠IgG二抗为固定相，游离CAP与生物素化的CAP人工抗原（CAP—BSA）共同竞争限量的CAP抗体；用铕标记的链霉亲和素示踪。[结果]该方法的灵敏度为0．016ng／ml。用于牛奶样品检测的添加回收率为89．6％，批内和批间变异系数分别为5．5％和10．1％，与甲砜霉素的交叉反应率〈0．01％。[结论]CAPTRFIA直接竞争法灵敏度高、特异性好、性能稳定，适于高通量筛查，有良好的应用前景。
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| 关 键 词： | 氯霉素 时间分辨荧光免疫分析法 牛奶 链霉亲和素 |
Determination of Chloramphenicol by Direct Competitive Time-resolved Fluoroimmunoassay |
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| ZHANG Yi et al. Determination of Chloramphenicol by Direct Competitive Time-resolved Fluoroimmunoassay[J]. Journal of Anhui Agricultural Sciences, 2009, 37(27): 12912-12914 |
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| Authors: | ZHANG Yi et al |
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| Affiliation: | ZHANG Yi et al(Jiangsu Institute of Nuclear Medicine,Wuxi,Jiangsu 214063) |
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| Abstract: | [Objective] A direct competitive time-resolved fluoroimmunoassay for determination of Chloramphenicol(CAP) was established.[Method] Coating with a goat-anti-mouse IgG,sample CAP competed with biotin-CAP-BSA to anti-CAP monoclonal antibody.It was traced with Eu-labeled streptavidin.[Result] The sensitivity of the assay was 0.016 ng/ml.Mean recovery for milk was 89.6%,with intra and inter assay variations 5.5% and 10.1%,respectively.The interference with thiamphenicol was less than 0.01%.[Conclusion] The CAP ... |
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| Keywords: | Chloramphenicol Time-resolved fluoroimmunoassay Milk Streptavidin |
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