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| 利用基因枪转化法获得受四环素调控的水稻雄性不育植株 | |
| 引用本文： | 田继微,廖江林,刘建丰,李文彬,肖国樱.利用基因枪转化法获得受四环素调控的水稻雄性不育植株[J].杂交水稻,2006,21(5):59-63. |
| 作者姓名： | 田继微 廖江林 刘建丰 李文彬 肖国樱 |
| 作者单位： | 1. 中国科学院,亚热带农业生态研究所,湖南,长沙,410125;湖南农业大学,湖南,长沙,410128 2. 中国科学院,亚热带农业生态研究所,湖南,长沙,410125 3. 湖南农业大学,湖南,长沙,410128 4. 中国科学院,遗传与发育生物学研究所,北京,100101 |
| 基金项目： | 中国科学院知识创新工程项目;湖南省杰出青年科学基金 |
| 摘 要： | 利用基因枪法通过pTET-BI-Bar和pTATx-BN-Bin质粒分别将TetR和Barnase基因共转化粳稻品种台北309、4008S和籼稻品种D68。通过对R0代植株不同的基因进行PCR及Southern分析,结果表明,Barnase、TetR基因和控制Barnase基因的TA29-TX启动子均已整合到水稻基因组。其中,台北309和4008S分别获得了15株和6株阳性转基因植株,其外源基因转化率分别为4.5%和1.9%,其外源基因TetR和Barnase的共转化率分别为0.6%和0.3%。D68没有获得阳性转基因植株。 |
| 关 键 词： | 水稻 基因枪转化 四环素调控的雄性不育基因 |
| 文章编号： | 1005-3956(2006)05-0059-05 |
| 收稿时间： | 2006-02-20 |
| 修稿时间： | 2006年2月20日 |
Developed Tetracycline-conditioned Male Sterile Transgenic Rice Plants by Particle Bombardment |
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| TIAN Ji-wei,LIAO Jiang-lin,LIU Jian-feng,LI Wen-bin,XIAO Guo-ying.Developed Tetracycline-conditioned Male Sterile Transgenic Rice Plants by Particle Bombardment[J].Hybrid Rice,2006,21(5):59-63. | |
| Authors: | TIAN Ji-wei LIAO Jiang-lin LIU Jian-feng LI Wen-bin XIAO Guo-ying |
| Institution: | 1. Institute of Subtropical Agrwalture, Chinese Academy of Sciences, Changsha, Hunan 410125, China; 2. Hunan Agricultural University, Changsha , Hunan 410128, China; 3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China |
| Abstract: | Taipei 309(japonica),4008S(japonica) and D68(indica) were co-transformed with Barnase and TetR genes via the plasmids of pTET-BI-Bar and pTATx-BN-Bin by particle bombardment.The PCR and southern blot analyses of each gene in R_0 plants showed that the Barnase,TetR genes and TA29-TX promoter were successfully integrated into the genome of transgenic plants.6 and 15 transgenic plants of 4008S and Taipei 309 were obtained,respectively.The gene transformation frequency of 4008S and Taipei 309 were 1.9% and 4.5%,respectively,and the efficiency of co-transformation of both TetR and Barnase genes of 4008S and Taipei 309 were 0.3% and 0.6%,respectively.However,no transgenic plants were found in the indica variety D68. |
| Keywords: | rice transformation by particle bombardment tetracycline-conditioned male sterile gene |
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