树舌灵芝中三萜类化合物的提取工艺研究

目的]研究树舌灵芝中三萜类化合物的提取工艺条件。方法]采用微波萃取法、浸提法、回流法和超声法研究了树舌灵芝中三萜类化合物的提取工艺条件,然后通过单因素试验和中心组合试验对提取工艺条件进行了优化,并比较了不同提取方法对三萜化合物的提取效率的影响。结果]微波萃取法的最佳提取条件为:乙醇浓度75%,提取时间15 min,提取温度75℃,料液比1∶20(g/ml);在此条件下三萜类化合物的平均提取率为0.748%;浸提法、回流法和超声法中三萜类化合物的提取率分别是微波萃取法的74%、80%和83%。结论]微波萃取法具有试剂用量少、时间短和提取效率高的优点,是树舌灵芝中三萜类化合物的最佳提取方法。

Study on the Extraction Technology of Triterpenoids from Ganoderma applanatum (Pers.) Pat.

Objective] To study the extraction technology of triterpenoids from Ganoderma applanatum(Pers.) Pat.Method] Microwave-assisted extraction(MAE),ordinary extraction,ultrasonic extraction and reflux extraction were used to study on triterpenoids from G.applanatum.By single factor experiment and orthogonal test,the extraction technology of triterpenoids was optimized.In addition,the effects of different methods on the extraction efficiency of triterpenoids were compared.Result] The optimal extraction technology of triterpenoids was as follows: G.applanatum were extracted with 75% ethanol at the solid-liquid ratio of 1∶20 g/ml for 15 min at 75 ℃.Under these conditions,the average extraction rate of triterpenoids was 0.748%.It showed that the extraction efficiency of ordinary extraction,reflux extraction and ultrasonic extraction were respectively 74%,80%,and 83% of microwave extraction by comparing the different methods.Conclusion] MAE is a better extraction method for the content determination of triterpenoids in G.applanatum that has the advantages of fewer solvent,shorter time and higher extraction efficiency.