超临界CO2萃取法与水蒸气蒸馏法提取香茅草挥发油化学成分比较 |
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引用本文: | 谢丽莎,龚志强,欧阳炜,黄振园. 超临界CO2萃取法与水蒸气蒸馏法提取香茅草挥发油化学成分比较[J]. 安徽农业科学, 2012, 40(20): 10397-10398 |
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作者姓名: | 谢丽莎 龚志强 欧阳炜 黄振园 |
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作者单位: | 广西中医学院赛恩斯新医药学院,广西南宁,530222;广西中医学院赛恩斯新医药学院,广西南宁,530222;广西中医学院赛恩斯新医药学院,广西南宁,530222;广西中医学院赛恩斯新医药学院,广西南宁,530222 |
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基金项目: | 广西壮族自治区教育厅科研项目 |
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摘 要: | [目的]比较不同方法提取的香茅草挥发油化学成分。[方法]采用超临界CO2流体萃取法(SCDE)及水蒸气蒸馏法(SD)从香茅草中提取挥发油,用气相色谱-质谱联用技术(GC-MS)对其化学成分进行定性定量分析。[结果]在超临界CO2流体萃取法提取的挥发油中共鉴定了31种成分,占挥发油总成分的91%以上;在水蒸气蒸馏法提取的挥发油中共鉴定了17种成分,占挥发油的94%以上。2种提取方法得到的挥发油组分及其含量差异较大,含量最高的都是香叶醛和橙花醛。[结论]超临界CO2流体萃取法提取的挥发油比水蒸气蒸馏法能更真实、全面的反映药材中的化学成分。
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关 键 词: | 香茅草[Cyrnbopogon citratus(DC.) Stapf] 超临界CO2流体萃取 水蒸汽蒸馏 挥发油 气相色谱-质谱 |
Comparison of Chemical Components in the Essential Oil Extracted by Supercritical CO2 Fluid and Steam Distillation from Cymbopogon citratus |
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Affiliation: | XIE Li-sha et al(Faculty of Chinese Medical Science,Guangxi Traditional Chinese Medical University,Nanning,Guangxi 530222) |
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Abstract: | [Objective] To compare the chemical components in the essential oil extracted by different methods from Cymbopogon citratus(DC.) Stapf.[Method] The essential oils were extracted by supercritical CO2 fluid and steam distillation.And then their chemical components were qualitatively and quantitatively analyzed by GC-MS.[Result] 31 components were identified in the essential oil extracted by supercritical CO2 fluid from Cyrnbopogon citratus,accounting for over 91% of the total volatile components;while 17 components were identified in the essential oil extracted by steam distillation,accounting for about 94% of the total components.The essential oil extracted by the two methods had different types and different contents of chemical components,geranial and neral were the most.[Conclusion] The essential oil extracted by supercritical CO2 fluid is better than that extracted by steam distillation to truly and comprehensively reflect the chemical components in medicines. |
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Keywords: | Cymbopogon citratus(DC.) Stapf SFE-CO2 Steam distillation Essential oil GC-MS |
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