仔猪大肠杆菌·C型产气荚膜梭菌菌液的培养工艺

[目的]研究仔猪大肠杆菌、C型产气荚膜梭菌菌液的培养工艺。[方法]分别试验不同的培养时间、培养温度和接种剂量对大肠杆菌纤毛表达量和C型产气荚膜梭菌产生毒素的影响。[结果]大肠杆菌在接种剂量为3%～5%,在36～37℃条件下培养7 h,可产生较高含量的纤毛抗原;当接种剂量为1%～2%,C型产气荚膜梭菌C59-2菌株在35℃条件下培养16～20 h,可产生大量致死性毒素。[结论]该研究为研制具有较好效果的仔猪红痢、黄痢预防疫苗提供了依据。

Study on Culture Technology of Escherichia coli and Clostridium Perfringens Type C of Piglets

[Objective] The aim was to study the culture technology of Escherichia coli and clostridium perfringens type C of piglets.[Method] Effects of different culture time,culture temperature and inoculation dosage on the cilia expression of E.coli and toxin production of clostridium perfringens type C were studied respectively.[Result] The results showed that the content of fimbriae antigen was higher when inoculation dosage of E.coli was 3%-5%,and cultured at 36-37 ℃ for 7 h.There would produce huge amounts lethal toxin when inoculation dosage of clostridium perfringens type C was 1%-2%,and cultured at 35 ℃ for 16-20 h.[Conclusion] The study provides a basis for development with good effect prevention vaccine of red scour of piglets and white scour of piglets.