| HPD-400大孔树脂分离纯化栀子黄色素的工艺研究 |
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| 引用本文： | 高丽,邓青云,姜益泉,李国元,张昭. HPD-400大孔树脂分离纯化栀子黄色素的工艺研究[J]. 安徽农业科学, 2012, 40(17): 9251-9253 |
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| 作者姓名： | 高丽 邓青云 姜益泉 李国元 张昭 |
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| 作者单位： | 孝感学院生命科学技术学院,湖北孝感,432000;孝感学院生命科学技术学院,湖北孝感,432000;孝感学院生命科学技术学院,湖北孝感,432000;孝感学院生命科学技术学院,湖北孝感,432000;孝感学院生命科学技术学院,湖北孝感,432000 |
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| 基金项目： | 湖北省自然科学基金重大项目 |
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| 摘 要： | [目的]研究大孔树脂分离纯化栀子黄色素的工艺条件。[方法]以9种大孔树脂为对象,考察其对栀子黄色素的吸附和洗脱性能的影响,筛选出适合吸附和分离黄色素的大孔树脂,并考察温度、酸度等因素对HPD-400吸附能力的影响,优选出分离纯化的最佳工艺条件。[结果]HPD-400对栀子黄色素的吸附能力最强,最佳吸附工艺条件为:温度25℃、pH为3.0、粗提液浓度A440 nm为0.590;最佳洗脱工艺条件为:乙醇浓度70%,温度25℃。[结论]HPD-400树脂分离纯化栀子色素的效果良好,可在工业上应用。
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| 关 键 词： | 栀子黄色素 大孔吸附树脂 吸附 解吸 |
Isolation and Purification of Gardenia Yellow Pigment by Macroporous Resin HPD-600 |
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| Affiliation: | GAO Li et al(College of Life Science and Technology,Xiaogan University,Xiaogan,Hubei 432000) |
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| Abstract: | [Objective] To study the absorption and purification conditions of Gardenia yellow pigment by macroporous resin.[Method] The best macroporous resin was screened out from nine resins according to their adsorption and elution performance of Gardenia yellow pigment.The influence of temperature,acidity,etc.on the absorption and desorption of HPD-400 was investigated to explore its best isolation and purification conditions.[Result] HPD-400 showed the largest adsorption capacity,its best adsorption conditions were 25 ℃ temperature,pH 3.0 and the concentration of crude extracts A440nm=0.590;the best elution conditions were 70% ethanol concentration and 25 ℃ temperature.[Conclusion] HPD-400 shows the best isolation and purification effects of Gardenia yellow pigment,thus can be used in industrialized production. |
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| Keywords: | Gardenia yellow pigment Macroporous resin Absorption Desorption |
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