不同条件对黄瓜发状根诱导率影响研究

以津研四号、新津春四号和玉蛟龙3种黄瓜为试材,通过比较影响发状根诱导率的条件,利用隶属函数综合评价农杆菌(A_4)对3种黄瓜发状根诱导能力,为建立一套高效的黄瓜发状根诱导体系提供依据。结果表明:3种黄瓜均成功诱导出发状根,转接培养后仍能正常生长。诱导条件分析表明,选择带叶茎段为外植体,菌液侵染10 min、菌液浓度为OD_(600)=0.3和乙酰丁香酮(acetosyringone)浓度为10 mg/L时诱导效果最好。隶属函数分析表明,A_4对3种黄瓜发状根诱导能力依次为玉蛟龙津研四号新津春四号。PCR分子鉴定表明,A_4菌Ri质粒的T-DNA基因已成功整合到3种黄瓜核基因组中。

Studies on Effects of Different Conditions on Induction Rate of Hairy Roots in Cucumis sativus L.

Taking 3 cultivars of cucumber “Jinyan 4”, “Xinjinchun 4” and “Yujiaolong” as experimental material, this study compared different conditions affecting induction rate of hairy root and comprehensively evaluated the induction abilities of hairy roots with A. rhizogenes (A4) among these 3 cucumber cultivers by the method of subordinate function. These studies would provide a high-efficiency system for the induction of agrobacterium rhizogenes in cucumber. Results showed that hairy roots were successfully induced in 3 cucumber cultivars, and they could grow normally after transferred. Analysis of different conditions showed that selections the stem with leaf as explant, 10 minutes as infection time, OD600=0.3 as microbial concentration and 10 mg/L as concentration of acetosyringone (As) were the optimized experiment conditions for induction rate of hairy roots. The order of induction ability on hair roots was “Yujiaolong”>“Jinyan 4”>“Xinjinchun 4” in these 3 cultivars of cucumber by subordinate function. It demonstrated that T-DNA on Ri plasmid of A4 had been integrated into the genome of nuclear cucumber genome successfully through molecular identification by PCR.