不同来源棉花种质资源遗传多样性的ISSR分析

 采取ISSR分子标记对48份棉花种质资源的遗传多样性进行分析，从60条ISSR引物中筛选出11条引物，这11条引物共扩增出92个条带，平均每条引物扩增出8.36个条带；其中多态性带77个条带，多态率达83.70％。UPGMA聚类分析显示，48份材料的相似性系数（GS）变化范围在0.27～0.93之间。聚类将48个种质资源划分为 4个大类(GS=0.55)，湛江野生棉、廉江野生棉与其它品种在遗传上有很大差别，属于较原始类型，归为一类；长绒棉与陆地棉品种存在明显的遗传差异也单独归为一类；其它不同省份的陆地棉品种在遗传上有较高的相似性，归为其它类型。分析结果表明，ISSR具有丰富遗传多样性和稳定性, 是一种较好的遗传分子标记，适宜于棉花品种遗传多样性分析

Genetic Diversity in Germplasm Resources of Cotton from Different Area Based on ISSR Markers

The genetic diversity of 48 cotton accessions were analyzed by using ISSR markers.11 primers were screened out of 60 primers. Total of 92 bands were detected, 77 of them were polymorphic. The percentage of polymorphic bands was 83.70%. UPGMA Cluster analysis based on ISSR data showed that the genetic similarity (GS)coefficient of 48 accessions ranged from 0.27 to 0.93. 48 germplasm resources could be categorized into four major groups, wild cotton of Zhanjiang and Lianjiang, Guangdong province, showed great genetic difference from other materials, fell into one group, belong to primitive group. G.barbadense cotton were obvious genetic diffrent from G.hirsutum, also fell into one group, and high similarity among the rest cultivars from different provinces formed other groups. The study indicated that ISSR molecular marker is an efficient tool with high polymorphism and stability for analysis the genetic diversity and relationship of cotton germplasm resources.