几种酶在大白菜软腐病抗性机制中的作用研究

测定不同抗性大白菜品种在不同生育期叶柄中软腐细菌含量和过氧化物酶、多酚氧化酶、蔗糖酶的活性。结果表明 ,从 2叶期到结球期 ,随生育期延长 ,感病品种细菌含量逐渐增高 ,抗病品种细菌含量保持不变 ;随生育期延长 ,抗病品种过氧化物酶活性逐渐升高 ,生育后期显著高于感病品种 ;不同抗性品种蔗糖酶活性均逐渐升高 ,但感病品种酶活性始终高于抗病品种 ;苗期抗病品种多酚氧化酶活性明显高于感病品种 ,生育后期多酚氧化酶活性均降低并且水平接近一致。客观地反应出在大白菜软腐病抗性机制中过氧化物酶起到抑制软腐细菌增殖作用 ,多酚氧化酶起到抑制侵染作用 ,蔗糖酶参与抗侵染、抗增殖作用

Study on effect of several enzymes in resistance mechanism of Soft Rotin Chinese Cabage

This study was to determine the content of Soft Rot bacteria and the activity of Peroxidase, Polyphendoxidase and sucrase of different resistant Chinese Cabbage during growing period .Experimental results indicated that bacteria content of susceptible caltivars increased gradually from 2th leaf age to head formation period with growing period was extended, but that of resistant cultivars was not changed ,Peroxidase activity of resistant caltivars increased gradully and was significantly higher than that of susceptible cultivers in the later stage of growing. Sucrase activity of different resistant cultivars increased gradully, but that of susceptible cultivars was higher than resistant ones all along. Polyphendoxidase activity of resistant cultivars was significantly higher than susceptible ones during seedling stage, and it descended and approach identical in the growing later stage. These results reflected objectively that peroxidase checked proliferation of bacteria, Polyphendoxidase checked infect and sucrase in volved in resistance of infect and proliferation in resistance mechanism of Soft Rot in Chinese Cabbage.$$$$