基因枪介导甘蔗遗传转化研究初报

将从灰树花（Grifola frondosa）中分离克隆到的海藻糖合酶cDNA以正向插入植物表达载体pBI121/BamHI Sst1位点，获得一植物表达载体pBT；又用Ubi-L启动子插入植物表达载体pBI121/HindⅢ＋Xbal位点，获得重组质粒pUB,再把海藻糖合酶cDNA正向插入pUB/BanHI SstI位点，获得另一植物表达载体pUBT。然后通过基因枪法分别用植物表达载体pBT和pUBT转化甘蔗。再生植株的点杂交和PCR－Southern杂交表明海藻糖合酶基因已整合进甘蔗的基因组中。

Genetic Transformation of Sugarcane Via Microprojectile Bombardment

Trehalose synthase (Tsase) cDNA isolated from Grifola frondosa was inserted into the sites of the expression vector pBI121/BamHI Sstl. This expression vector was called PBT. The Ubi-L promoter was first inserted into the sites of the expression vector pBI121/HindIII Xbal, and the recombinant plasmid pUB was obtained. The Tsase gene was then inserted into the sites of the plasmid pUB/BamHI Sstl. Hence the expression vector pUBT was constructed. The pBT and pUBT were then transferred into sugarcane via microprojectile bombardment. The dot-blot hybridization and PCR-Southern analysis demonstrated that the Tsase gene was integrated into sugarcane genomes.