| 棉花Oligo-FISH技术建立及其初步应用 |
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| 引用本文： | 刘玉玲,刘震,李兆国,王玉红,周忠丽,蔡小彦,王星星,王小艳,张树林,赵海燕,张振梅,王坤波,刘方,彭仁海.棉花Oligo-FISH技术建立及其初步应用[J].棉花学报,2017,29(3):213-221. |
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| 作者姓名： | 刘玉玲 刘震 李兆国 王玉红 周忠丽 蔡小彦 王星星 王小艳 张树林 赵海燕 张振梅 王坤波 刘方 彭仁海 |
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| 作者单位： | 1. 安阳工学院,河南安阳455000;中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000;2. 安阳工学院,河南安阳,455000;3. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳,455000 |
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| 基金项目： | 国家自然科学基金(31471548)，棉花生物学国家重点实验室开放课题(CB2017A06)，安阳工学院博士科研启动基金(BSJ2016005) |
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| 摘 要： | 【目的】荧光原位杂交技术可以实现DNA序列直观准确的染色体定位,是基因组深入研究的重要技术之一。染色体特异探针的获得是该技术应用的关键。本研究旨在建立棉花寡核苷酸荧光原位杂交技术。【方法】利用已经公布棉花基因组序列数据,采用生物信息学方法获得染色体特异的寡核苷酸库,随后用乳化聚合酶链式反应方法标记成荧光探针,在棉花有丝分裂中期染色体上进行原位杂交。【结果】建立了一套棉花寡核苷酸荧光原位杂交技术体系。【结论】该体系可用于棉花单染色体识别鉴定。
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| 关 键 词： | 棉花 寡核苷酸 荧光原位杂交 染色体 |
Construction and Primary Application of Oligos Fluorescence in situ Hybridization Technology in Cotton |
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| Liu Yuling,Liu Zhen,Li Zhaoguo,Wang Yuhong,Zhou Zhongli,Cai Xiaoyan,Wang Xingxing,Wang Xiaoyan,Zhang Shulin,Zhao Haiyan,Zhang Zhenmei,Wang Kunbo,Liu Fang,Peng Renhai.Construction and Primary Application of Oligos Fluorescence in situ Hybridization Technology in Cotton[J].Cotton Science,2017,29(3):213-221. |
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| Authors: | Liu Yuling Liu Zhen Li Zhaoguo Wang Yuhong Zhou Zhongli Cai Xiaoyan Wang Xingxing Wang Xiaoyan Zhang Shulin Zhao Haiyan Zhang Zhenmei Wang Kunbo Liu Fang Peng Renhai |
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| Institution: | 1. Anyang Institute of Technology, Anyang, Henan 455000, China;2. Institute of Cotton Research of Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China |
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| Abstract: | Objective] Fluorescence in situ hybridization (FISH) can achieve the accurate chromosomal localization of DNA sequences,which is one of the important techniques for genome in-depth study.The acquisition of chromosome-specific probes is the key for FISH application.The aim of this study is to explore a system of cotton oligonucleotides (oligos) fluorescence in situ hybridization (Oligo-FISH) technology.Method] Based on the published cotton genome sequence,the chromosome-specific oligos library was obtained by bioinformatics method.Then,the obtained oligos were labeled through emulsion polymerase chain reaction,and used for FISH of cotton mitotic chromosome.Results] A system of cotton Oligo-FISH technology was set up and successfully applied in cotton chromosome identification.Conclusion] This system can be used for dissection of cotton genomes. |
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| Keywords: | cotton oligos fluorescence in situ hybridization chromosome |
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