华盛顿脐橙果实采后油胞病害与酶活性的相关性

以华盛顿脐橙果实为试验材料,研究采后果皮油胞病害的发生状况及病变过程中果皮POD(过氧化物酶)、PPO(多酚氧化酶)、PAL(苯丙氨酸解氨酶)等相关酶活性的变化,以探索柑橘果皮油胞的病变生理。研究结果表明,果皮油胞病害发生率随贮藏时间的增加而上升;伤果的发病率显著增加,发病时间早;低温降低了贮藏后期果皮油胞病变率。损伤处理显著提高了果皮PPO、POD和PAL酶活性,而低温则抑制了这些酶活性。在整个常温贮藏过程中,正常果和损伤果的油胞病害发生率与这3种酶活性都呈显著和极显著正相关。说明果皮POD、PPO和PAL的高活性是果皮油胞病变的重要生理因素。

Study on the Relationship between Enzymatic Activity and Oil Spotting (Oleocellosis) Infection of Postharvest Washington Navel Orange Peel

In order to clarify the Oil Spotting physiological pathogenesis of citrus fruit peel,Washington Navel Orange in storage period was used to investigate the oil spotting infection and the activity of PPO?POD and PAL in the peel were tested. The results showed that the blotched fruit ratio caused by oil spotting increased with time of storage,but reduced by cold storage especially in later period. The wounded treatment accelerated pathological change and caused earlier occurrence than the contrast. The activity of PPO?POD and PAL in the wounded fruit peel increased significantly,and reduced by cold storage treatment. During the room temperature storage period,the fruit ratio of oil spotting-infected all correlated significantly and positively to the activity of PPO?POD and PAL in the wounded and normal fruit peel. So, it conclude that the activities of PPO?POD and PAL in the fruit peel were important physiological factors in oil spotting infection.