茶树密码子用法分析

运用CodonW软件和CUSP程序对筛选的134个茶树蛋白质编码基因序列进行了分析、计算密码子使用频率,并将它与人、果蝇、酵母、大肠杆菌这4种代表性生物及拟南芥、大豆、棉花、水稻、小麦等单、双子叶植物进行比较。结果显示茶树密码子偏爱性与人、果蝇、酵母、大肠杆菌有不同程度的差异,与单子叶植物水稻、小麦的密码子使用频率差异较大,与双子叶植物拟南芥、大豆、棉花的密码子偏爱性一致,分析结果对茶树基因转化及高效表达系统的选择等具有重要指导意义。

Analysis of Codon Usage in Tea Plant (Camellia sinensis)

The coding sequence of 134 selected proteins in tea plant(Camellia sinensis) were analyzed by CodonW and CUSP program in this investigation,and the frequency of codon usage which encoded amino acids was calculated and compared with four classes of representative organisms including human(Homo sapiens),yeast(Saccharomyces cerevisiae),Escherichia coli.Bacteria and fruit fly(Drosophila melanogaster).These results showed that codon preference of tea plant was differed in various degrees with these model organisms.Accomparison of codon usage frequency of tea plant with dicotyledons and monocotyledons plants including Arabidopsis thaliana,soybean(Glycine max),cotton(Gossypium hirsutum),rice(Oryza sativa),wheat(Triticum aestivum),the consequence indicated that codon usage frequency of tea plant was obviously differed from these monocotyledons such as Oryza sativa and Triticum aestivum,but had a consistency in codon preference with these dicotyledons such as Arabidopsis thaliana,Glycine max and Gossypium hirsutum.These results showed an important guidance for tea plant gene-transforming and the selection of the high-efficient expression system.