高邮鸭PRL基因内含子1的SNP检测及其与产蛋性状的相关分析

利用PCR—RFLP技术对高邮鸭催乳素（Prolactin，PRL）基因内含子1进行了SNP检测和测序分析，并对该基因与高邮鸭产蛋性状的相关性进行了研究。结果表明：PRL基因内含子1存在两个Dra Ⅰ酶切位点，其中1个位点具有DraⅠ多态性。该位点由于在1326位点发生了T→C的碱基突变，产生了AA、AB和BB3种基因型。基因型BB和等位基因B的频率最高；BB基因型个体30周龄蛋重极显著高于AB基因型个体（P〈0．01）；AB基因型个体的双黄率显著高于BB基因型个体（P〈0．05）；3种基因型间产蛋数、最长连产天数和开产体重无显著差异。

SNP Detection of Intron 1 of PRL Gene and Its Associations with Egg-laying Traits in Gaoyou Duck

In present study,SNP of prolactin(PRL) gene intron 1 of Gaoyou duck were detected by PCR-RFLP and DNA sequencing.The correlation analysis between the polymorphism of PRL gene intron 1 and egg-laying traits was carried out.The results indicated that there were two Dra I digestion sites in intron 1 of PRL gene,but only one was polymorphic.A T/C mutation at the position of the 1 326 bp of the PRL gene was found by DNA sequencing,and resulted in 3 genotypes(AA,AB and BB).The frequencies of genotype BB and allele B were the highest.The least square analysis showed that the BB ducks had higher egg weight at the age of 30 weeks than AB ducks(P<0.01),the proportion of double-yolk of the AB ducks was higher than that of BB ducks(P<0.05),but there was not significant difference in egg number,the longest days of continual laying and the body weight at first laying egg among 3 genotypes.