| 密度对黑腹果蝇胚细胞系L-2/M delta 2-3 冻存效果的影响 |
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| 引用本文： | 丁伟峰,冯颖,张欣,李娴.密度对黑腹果蝇胚细胞系L-2/M delta 2-3 冻存效果的影响[J].林业科学研究,2012,25(1):6-10. |
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| 作者姓名： | 丁伟峰 冯颖 张欣 李娴 |
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| 作者单位： | 中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明650224 |
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| 基金项目： | 林业公益性行业科研专项(4-38) |
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| 摘 要： | 使用双翅目昆虫黑腹果蝇( Drosophila melanogaster Fallen )胚细胞系L-2/M delta 2-3作为研究材料,制备10个密度试验组(0.81×106~2.88×107 个·mL-1,每组跨度大于2.00×106 个·mL-1),分别在冻后第6、10、14个月对各组细胞冻后活力、回复时间长短、以及生长状况进行观察和比较,研究密度因素对细胞系L-2/M delta 2-3长期冻存效果的影响。结果表明:冻存密度对细胞系L-2/M delta 2-3冻后活力和状态恢复均有显著影响( P<0.05 )。细胞冻后活力下降速率随冻存密度增高而减慢,复苏后细胞状态恢复所需时间较短。将L-2/M delta 2-3的冻存密度提高至1.3×107 个·mL-1以上有利于细胞活力的保持与冻后的恢复。
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| 关 键 词： | 冻存密度 冻存时间 昆虫细胞 细胞活力 |
| 收稿时间： | 2011/9/12 0:00:00 |
Cryopreservation of Cell Line L-2/M delta 2-3 from Embryo of Drosophila melanogaster:The Effects of Cell Density on the Cytoactive |
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| DING Wen-feng,FENG Ying,ZHANG Xin and LI Xian.Cryopreservation of Cell Line L-2/M delta 2-3 from Embryo of Drosophila melanogaster:The Effects of Cell Density on the Cytoactive[J].Forest Research,2012,25(1):6-10. |
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| Authors: | DING Wen-feng FENG Ying ZHANG Xin and LI Xian |
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| Institution: | Research Institute of Resource Insects, Chinese Academy of Forestry; Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry; Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry; Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry; Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China |
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| Abstract: | In order to evaluate the effects of different cell density on post-thaw cytoactives ( including cell viability, recovery time, etc.) of insect cell line L-2/M delta 2-3 from embryo of Drosophila melanogaster Fallen, 10 test groups with different cell density (from 0.81×106 cells·mL-1 to 2.88×107 cells·mL-1, the density span of each group was more than 2.00×106 cells·mL-1 ) were prepared as the cryopreserved cultures preserved in liquid nitrogen separately during 14 months. The results showed that the post-thaw viability of each test group decreased with the cryopreservation time expended. The rate of post-thaw viability decline decreased with the cell density of cryopreserved cultures increased. Cultures frozen at above 1.3×107 cells·mL-1 could obtain a better effect of cryopreservation. |
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| Keywords: | cell density cryopreserveing duration insect cells cell viability |
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