| 猪圆环病毒病和猪繁殖与呼吸综合征二联灭活疫苗免疫效力 |
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| 引用本文： | 金扩世,韩松,鲁会军,李洋,杨尧,李昌,李霄,田明尧,金宁一.猪圆环病毒病和猪繁殖与呼吸综合征二联灭活疫苗免疫效力[J].中国兽医学报,2010,30(5). |
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| 作者姓名： | 金扩世 韩松 鲁会军 李洋 杨尧 李昌 李霄 田明尧 金宁一 |
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| 作者单位： | 军事医学科学院军事兽医研究所,吉林长春,130062 |
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| 基金项目： | 吉林省科技发展计划重点资助项目(20080216) |
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| 摘 要： | 利用猪圆环病毒病(PCVD)和猪繁殖与呼吸综合征(PRRS)二联灭活疫苗免疫4周龄仔猪,免疫后7、14、21、28d采血,测定免疫后血清中抗PRRSV、PCV中和抗体和ELISA抗体较价,并于免疫后28d分别用PRRSV和PCV2攻毒,攻毒后每日检测猪体温和观察临床症状,于攻毒后21d所有存活猪全部扑杀,分别对每头猪肺、肝、脾、淋巴结、心和血进行剖解病理和组织病理学观察及PRRSV和PCV2核酸检测,以确定该疫苗免疫保护率。结果表明,所有试验猪于免疫后7d血清抗体开始检出,28d达到峰值,免疫后28dPRRSV和PCV2攻毒保护率均为100%(5/5)。
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| 关 键 词： | PRRSV PCV2 二联灭活疫苗 效力试验 |
Protective efficiency tests of the inactivated PCV2 and PRRS vaccine |
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| JIN Kuo-shi,HAN Song,LU Hui-jun,LI Yang,YANG Yao,LI Chang,LIi Xiao,TIAN Ming-yao,JIN Ning-yi.Protective efficiency tests of the inactivated PCV2 and PRRS vaccine[J].Chinese Journal of Veterinary Science,2010,30(5). |
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| Authors: | JIN Kuo-shi HAN Song LU Hui-jun LI Yang YANG Yao LI Chang LIi Xiao TIAN Ming-yao JIN Ning-yi |
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| Institution: | JIN Kuo-shi,HAN Song,LU Hui-jun,LI Yang,YANG Yao,LI Chang,LIi Xiao,TIAN Ming-yao,JIN Ning-yi (Military Veterinary Institute,Academy of Military Medical Sciences,Changchun 130062,China) |
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| Abstract: | Efficiency tests of inactivated porcine circovirus dieases(PCVD) and porcine reproductive and respiratory syndrome virus (PRRS) vaccine were detected by serum neutratiziation(SN) and enzyme-linked immunosorbent assay (ELISA) on 7,14,21,28 day post-vaccinated 4-week piglets.Changes of body temperature and clinical symptoms of piglets were detected on 28 day post vaccination.The vaccinated piglets were killed 21 days post vaccination,and dissection,histological were examined and PCV2 and PRRSV genes were ampl... |
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| Keywords: | PRRSV PCV2 inactivated vaccine protective efficacity |
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