| 杨梅种间杂交及杂种F_1的胚培养 |
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| 引用本文： | 谢小波,求盈盈,郑锡良,戚行江,邱立军,黄忠平,王涛,梁森苗. 杨梅种间杂交及杂种F_1的胚培养[J]. 果树学报, 2009, 26(4) |
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| 作者姓名： | 谢小波 求盈盈 郑锡良 戚行江 邱立军 黄忠平 王涛 梁森苗 |
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| 作者单位： | 谢小波,求盈盈,郑锡良,戚行江,梁森苗,XIE Xiao-bo,QIU Ying-ying,ZHENG Xi-liang,QI Xing-jiang,LIANG Sen-miao(浙江省农业科学院园艺研究所,杭州,310021);邱立军,QIU Li-jun(浙江省定海区农业林业局,浙江舟山,316000);黄忠平,HUANG Zhong-ping(杭州市萧山区所前镇农业办公室,浙江萧山,325000);王涛,WANG Tao(温岭市农业林业局,浙江温岭,317500) |
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| 基金项目： | 浙江省科技计划项目，浙江省重大科技专项项目，浙江省留学回国人员择优资助项目 |
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| 摘 要： | 在杨梅属的杨梅种和矮杨梅种之间进行了人工远缘杂交,然后对其杂交F1代成熟种子作了胚培养研究,并用SSR分子标记对杂种的真实性进行了鉴定。结果表明,杨梅种和矮杨梅种有着较好的杂交亲和性,以杨梅种为母本、矮杨梅种为父本的F1杂交种中,70.5%的种子胚正常发育;以矮杨梅种为母本、杨梅种为父本的F1杂交种中,76.5%的种子胚发育正常。用MS添加0.5mg·L-1的6-BA培养基对矮杨梅种(♀)×杨梅种(♂)和杨梅种(♀)×矮杨梅种(♂)2个F1杂交组合以及杨梅品种晚稻杨梅(WD)、迟色(CS)等4份材料的胚培养中,绿苗诱导率分别为85.0%、28.6%、6.9%、0.0。在1/2MS添加0.5mg·L-1IBA的生根培养基中,矮杨梅种(♀)×杨梅种(♂)和杨梅种(♀)×矮杨梅种(♂)F1绿苗的生根率分别为29.2%和33.3%。SSR分子标记对杨梅种(♀)×矮杨梅种(♂)F1的鉴定表明杂交后代为真杂种。研究结果为杨梅育种提供了新的途径,并为杨梅遗传连锁作图研究的群体构建奠定了技术基础。
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| 关 键 词： | 杨梅 矮杨梅 种间杂交 杂种F1 胚培养 |
Studies on the crossing between Myrica rubra and M. nana and embryo culture in vitro of its hybrid F_1 |
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| XIE Xiao-bo,QIU Ying-ying,ZHENG Xi-liang,QI Xing-jiang,QIU Li-jun,HUANG Zhong- ping,WANG Tao,LIANG Sen-miao. Studies on the crossing between Myrica rubra and M. nana and embryo culture in vitro of its hybrid F_1[J]. Journal of Fruit Science, 2009, 26(4) |
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| Authors: | XIE Xiao-bo QIU Ying-ying ZHENG Xi-liang QI Xing-jiang QIU Li-jun HUANG Zhong- ping WANG Tao LIANG Sen-miao |
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| Affiliation: | 1Institute of Horticulture;Zhejiang Academy of Agricultural Sciences;Hangzhou;Zhejiang 310021 China;2Department of Agriculture and Forestry;Dinghai County;Zhejiang 316000 China;3Suoqian Agricultural Office;Xiaoshan District;Zhejiang 325000 China;4Department of Agriculture and Forestry;Wenling County;Zhejiang 317500 China |
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| Abstract: | Crossing was made between Myrica rubra and Myrica nana, then embryo culture in vitro of the F1 seeds was studied,and seedlings were tested with SSR marker technology for hybrid authenticity. Results showed that there was a good cross compatibility between M. rubra and M. nana. The ratio of normal seeds with intact embryo was 70.5% when M. rubra was used as female and M. nana as male parent; and 76.5% when M. rubra was used as male parent. During embryo culturing in vitro with the classic salt solution of Mu... |
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| Keywords: | Myrica rubra M. nana Inter-specific crossing Hybrid F1 Embryo culture |
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