罗非鱼鱼皮酸溶性胶原蛋白的制备工艺研究简 |
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引用本文: | 黄敏,陈海光,刘朝霞,黄东雨. 罗非鱼鱼皮酸溶性胶原蛋白的制备工艺研究简[J]. 安徽农业科学, 2013, 0(22): 9427-9430 |
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作者姓名: | 黄敏 陈海光 刘朝霞 黄东雨 |
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作者单位: | [1]广东科贸职业学院,广东广州510430 [2]仲恺农业工程学院轻工食品学院,广东广州510225 [3]广东检验检疫技术中心,广东广州510623 [4]从化职业技术学院,广东从化510900 |
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摘 要: | [目的]研究从罗非鱼鱼皮中用酸溶剂提取胶原蛋白的最佳条件.[方法]以罗非鱼鱼皮为原料,利用酸处理提取胶原蛋白.以提取溶液、浓度、浸提时间、料液比为因素,通过测定其羟脯氨酸含量,计算不同提取条件下胶原蛋白的得率.再利用响应面法对提取条件进行优化,对拟合多项式回归模型进行分析.[结果]由响应面优化得到罗非鱼鱼皮中胶原蛋白最佳酸提取工艺条件为:乙酸浓度0.53 mol/L,浸泡时间64.8h、料液比1∶25 g/ml,此条件下胶原蛋白提取量试验均值为244.86 mg/g.[结论]研究可为罗非鱼鱼皮中胶原蛋白的提取利用提供参考.
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关 键 词: | 罗非鱼鱼皮 胶原蛋白 酸提取 工艺优化 |
Study on the Extraction of Acid-solubl College from Oreochromis nilofscus Skin |
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Affiliation: | HUANG Min et al (Guangdong Vocational College of Science and Trade, Guangzhou, Guangdong 510430) |
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Abstract: | [ Objective ] To study the optimum conditions of extracting collagen using acid solution from the skin of Tilapia. [ Method ] The ex- tracting technology of collagen using acid solution from the skin of Tilapia Was studied in this paper, Choosing acid type, concentration of acid solution extraction time and solid-liquid ratio as single factor experiment factors, :the extracting results were achieved by measuring the hydroxyproline content, the yield of collagen under different extraction conditions were calculated. The optimal extracting conditions were in- vestigated based on box-behnken design and response surface methodology. [ Result] By analyzing the fitted polynomial regression model, the optimum parameters of extracting collagen from the skin of Tilapia were as follows : soaking Tilapia skin in acetic acid solution 0.53 mol/L with solid-liquid ratio 1:25 g/ml at 4 ℃ for 64.8 h, and the optimized extraction led to a collagen content of 244.86 mg/g. [ Conclusion] The study will provide reference for extraction and utilization of collagen from skin of Tilapia. |
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Keywords: | Skin of Tilapia Collagen Extraction in acid solution Process optimization |
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