Sterol oxidation in ready-to-eat infant foods during storage |
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Authors: | García-Llatas Guadalupe Cercaci Luisito Rodriguez-Estrada Maria Teresa Lagarda M Jesús Farré Rosaura Lercker Giovanni |
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Affiliation: | Area de Nutrición y Bromatología, Universitat de València, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot-Valencia, Spain. |
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Abstract: | The effect of storage on sterol oxidation of ready-to-eat infant foods was evaluated. Two different liquid infant foods (honey or fruits flavors), prepared with milk and cereals, were stored for 0, 2, 4, 7 and 9 months at 25 degrees C. Sterol oxidation products (SOP) were isolated by cold saponification, purified by silica solid-phase extraction, and analyzed by gas chromatography (GC) and GC-mass spectrometry. beta-Sitosterol was the most representative sterol, followed by cholesterol and campesterol. No significant differences in the total and single SOP content (0.8-1 mg/kg of product) were observed with respect to storage time and type of sample; the main SOP found was 7-ketositosterol (<0.2 mg/kg of product). The extent of stigmasterol oxidation (2.9%) was higher than that of cholesterol (1.9%) and beta-sitosterol (1.4%). The type and quality of raw materials, as well as the processing conditions, seem to greatly influence SOP formation and accumulation in infant foods. |
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