枇杷胚性培养物ACC氧化酶基因的克隆及序列分析

以枇杷胚性培养物（Eriobotrya japonica embryonic cultures）为材料，采用同源克隆方法，从其mRNA中分离得到ACC氧化酶（ACO）基因EjACO-1，在GenBank中登录号为GQ377219。结果表明：EjACO-1大小为 1 160 bp，编码322个氨基酸。枇杷EjACO-1与苹果、桃和梨等蔷薇科植物同源性较高，而与水稻、石斛兰等单子叶植物距离较远。此外，从枇杷基因组DNA中分离了转录为EjACO-1 mRNA的基因gACO，大小为1 517 bp，在GenBank中的登录号为GU233743。生物信息学分析显示，gACO基因中含有3个内含子，大小分别为114、240、194 bp，均符合真核生物内含子通用的GT-AG法则。EjACO基因的克隆为以后研究乙烯在枇杷中的功能奠定基础。

Cloning and Sequence Analysis of ACC Oxidase Gene from Eriobotrya japonica Embryonic Cultures

Homologous cloning method was used to clone the 1-aminocyclopropane-1-carboxylic acid oxidase(ACO)gene,EjACO-1,Eriobotrya japonica embryonic cultures.The gene,GenBank accession number GQ377219,was of 1 160 bp,encoding 322 amino acids,and highly homologous to other rosaceous plants origins(Malus x domestica,Prunus persica,Pyrus pyrifolia and so on),and was distant from that of monocotyledons(Oryza sativa,Dendrobium and so on).Moreover,gACO,which encoding EjACO-1 mRNA,was obtained from the genomic DNA.The length of gACO was 1 517 bp,and the accession number was GU180353 in GenBank.The gACO gene contained 3 introns consistent with the GT-AG dogma for eukaryotic introns,with lengths 114,240 and 194 bp,respectively.The cloning of EjACO provides a foundation for further study of ethylene function in Eriobotrya japonica.