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| 非编码RNA BcNR1在菜心中的克隆及表达分析 | |
| 引用本文： | 宋江华,赵颖,汪承刚,曹家树.非编码RNA BcNR1在菜心中的克隆及表达分析[J].热带作物学报,2012,33(6):1073-1076. |
| 作者姓名： | 宋江华 赵颖 汪承刚 曹家树 |
| 作者单位： | 1. 安徽农业大学园艺学院,安徽合肥230036;浙江大学蔬菜研究所,浙江杭州 310029 2. 安徽农业大学园艺学院,安徽合肥,230036 3. 浙江大学蔬菜研究所,浙江杭州,310029 |
| 基金项目： | 国家自然科学基金项目（No. 30800750），安徽省自然科学基金项目（No. 10040606Q20）。 |
| 摘 要： | 非编码RNA是近年来新发现的一类在生物体内广泛存在的特殊基因.以菜心Brassica campestris L.ssp.chinensis(L.)Makino vat.utilis]为实验材料,根据普通白菜花粉特异的非编码RNA基因Bc MF11的全长序列设计引物,通过PCR直接扩增的方法从菜心中克隆出BcMF11的同源基因全长序列,命名为BcNR1 (GenBank登录号为EF363720).序列分析显示,该基因全长801 bp,缺乏明显的开放阅读框,而且在序列中多处出现终止密码子,具有非编码RNA基因的序列特征.RT-PCR表达分析结果显示BcNR1是菜心花粉发育特异表达的基因,推测该非编码RNA基因在植物花粉发育中发挥作用. |
| 关 键 词： | 菜心 BcNR1 非编码RNA 基因克隆 表达分析 |
Cloning and Expression of Non-coding RNA BcNR1 in Brassica campestris L. var. utilis |
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| SONG Jianghu,ZHAO Ying,WANG Chenggang and CAO Jiashu.Cloning and Expression of Non-coding RNA BcNR1 in Brassica campestris L. var. utilis[J].Chinese Journal of Tropical Crops,2012,33(6):1073-1076. | |
| Authors: | SONG Jianghu ZHAO Ying WANG Chenggang and CAO Jiashu |
| Institution: | 1 College of Horticulture, Anhui Agricultural University 2 Institute of Vegetable Science, Zhejiang University;College of Horticulture, Anhui Agricultural University;College of Horticulture, Anhui Agricultural University;Institute of Vegetable Science, Zhejiang University |
| Abstract: | Non-coding RNA is a group of new special RNA widely existed in many organisms. In this study, Brassica campestris L. ssp. chinensis(L.)Makino var. utilis was used as the experimental material. BcNR1 gene, homologous to the BcMF11 encoding pollen-specific non-coding gene of Chinese cabbage, was cloned from Brassica campestris L. var. utilis by PCR amplification. BcNR1 had a total length of 801 bp. Sequence analysis revealed that BcNR1 is a novel non-coding RNA containing a high density of stop codons and lacking extensive open reading frame. RT-PCR analysis showed that BcNR1 was highly expressed in anthers. It means that the non-coding RNA may act in pollen development. |
| Keywords: | Brassica campestris var utilis BcNR1 non-coding RNA Gene cloning Expression analysis |
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