| 香蕉嗜铁内生细菌BEB99的分离鉴定及其抗病促生能力评价 |
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| 引用本文： | 戴英葵,刘先宝,张科立,蔡吉苗,黄贵修.香蕉嗜铁内生细菌BEB99的分离鉴定及其抗病促生能力评价[J].热带作物学报,2012,33(5):913-918. |
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| 作者姓名： | 戴英葵 刘先宝 张科立 蔡吉苗 黄贵修 |
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| 作者单位： | 1. 海南大学,海南海口 570228;中国热带农业科学院环境与植物保护研究所 农业部热带作物有害生物综合治理重点实验室 海南省热带农业有害生物监测与控制重点实验室 海南省热带作物病虫害生物防治工程技术研究中心 海南儋州 571737
2. 中国热带农业科学院环境与植物保护研究所 农业部热带作物有害生物综合治理重点实验室 海南省热带农业有害生物监测与控制重点实验室 海南省热带作物病虫害生物防治工程技术研究中心 海南儋州 571737 |
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| 基金项目： | 科技部国家科技支撑计划项目子课题目（No． 2007BAD48B05；No． 2009hzs1J005）；科技部科技人员服务企业项目“香蕉健康种苗标准化生产技术的应用及推广”（No．2009GJE20046；No．2009GJE20046）；海南大学“211工程”建设项目；中国热带农业科学院博士启动经费“未培养香蕉内生细菌类群多态性分析及其功能验证”（Hzs0601）。 |
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| 摘 要： | 从健康的香蕉根组织内分离获得一株对香蕉枯萎病菌（Fusarium oxysporum f.sp. cubense）有拮抗作用的内生细菌菌株BEB99，经形态学、生理生化特征及16S rDNA序列同源性分析等方面的研究，鉴定该菌为劳尔氏菌（Ralstonia sp.）。铁载体检测结果发现，该菌株属于极高产铁载体菌株。盆栽试验结果表明，该菌株对香蕉枯萎病的防治效果达62.52％，并对香蕉植株具有显著的促生作用。
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| 关 键 词： | 香蕉 内生细菌 铁载体 抗病促生能力 |
Isolation and Identification of Endophytic Bacterium Strain BEB99 from Banana and Evaluation of Its Antagonistic
Activity and Growth-promoting Ability |
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| DAI Yingkui,LIU Xianbao,ZHANG Keli,CAI Jimiao and HUANG Guixiu.Isolation and Identification of Endophytic Bacterium Strain BEB99 from Banana and Evaluation of Its Antagonistic
Activity and Growth-promoting Ability[J].Chinese Journal of Tropical Crops,2012,33(5):913-918. |
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| Authors: | DAI Yingkui LIU Xianbao ZHANG Keli CAI Jimiao and HUANG Guixiu |
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| Institution: | 1 Hainan University
2 Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Hainan Key Laboratory
for Monitoring and Control of Tropical Agricultural Pests, Hainan Engineering Research Center for Biological
Control of Tropical Crops Diseases and Insect Pests, P.R.China, CATAS;Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Hainan Key Laboratory
for Monitoring and Control of Tropical Agricultural Pests, Hainan Engineering Research Center for Biological
Control of Tropical Crops Diseases and Insect Pests, P.R.China, CATAS;Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Hainan Key Laboratory
for Monitoring and Control of Tropical Agricultural Pests, Hainan Engineering Research Center for Biological
Control of Tropical Crops Diseases and Insect Pests, P.R.China, CATAS;Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Hainan Key Laboratory
for Monitoring and Control of Tropical Agricultural Pests, Hainan Engineering Research Center for Biological
Control of Tropical Crops Diseases and Insect Pests, P.R.China, CATAS;Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Hainan Key Laboratory
for Monitoring and Control of Tropical Agricultural Pests, Hainan Engineering Research Center for Biological
Control of Tropical Crops Diseases and Insect Pests, P.R.China, CATAS |
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| Abstract: | Strain BEB99,an endophytic bacterium exhibited strong inhibitory activity in vitro against banana wilt fungus,was isolated from the root of Musa AAA,cv.Baxi.This strain was identified as Ralstonia sp.based on its morphological,physiological and biochemical characteristics and its 16S rDNA sequences analysis.Strain BEB99 could produce siderophore and significantly promoted the growth of banana.Pot experiments indicated that the strain could suppress Fusarium wilt disease,with an inhibitory rate of 62.52%. |
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| Keywords: | Banana Endophytic bacteria Siderophore Growth-promoting ability |
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