漳州水仙花瓣和副冠类胡萝卜素成分分析及合成途径基因表达研究

采用高效液相色谱（HPLC）分析漳州水仙‘金盏银台’花瓣和副冠中主要类胡萝卜素成分。结果显示，黄色副冠的主要色素成分是叶黄素，含量为3.73×104 μg/g ，β-胡萝卜素的含量很低，仅2.7 μg/g；白色花瓣中各种类胡萝卜素的含量都极低。同时，采用RT-PCR方法分析类胡萝卜素合成途径结构基因PSY、PDS、ZDS、LYCE、LYCB、BCH在‘金盏银台’白色花瓣、黄色副冠，洋水仙‘粉红魅力’白色花瓣、红色花副冠，洋水仙‘嘹亮’黄色花瓣、橙黄色副冠中的表达情况。结果表明，花瓣与同一品种副冠间结构基因

Carotenoid Pigment Components and Carotenogenic Gene Expression in Zhangzhou narcissus(Narcissus tazetta var.chinensis)

To evaluate the carotenoid components,the extracts from perianths and coronas of Zhangzhou narcissus(Narcissus tazetta var.chinensis) ’Jinzhanyintai’ were analyzed separately by HPLC.The results showed that the most abundant carotenoid pigment in yellow corona was lutein,3.73×104 μg/g in content and the content of β-carotene was very low,only 0.00027 ×104 μg/g.The content of each carotenoid component was little in white perianths of Zhangzhou narcissus.The expression of carotenoid biosynthetic genes in Zhangzhou narcissus,Narcissus pseudonarcissus ’ Pink charm’ and ’ Fortissimo’ was also investigated,including phytoene synthase(PSY),phytoene desaturase(PDS),ζ-carotene desaturase(ZDS),lycopene β-cyclase(LYCB),lycopene e-cyclase(LYCE) and β-carotene hydroxylase(BCH).The similar expression between perianths and coronas of the same variety and changes in the expression of PDS,ZDS,LYCE and BCH between ’ Pink charm’ and other two varieties were observed.These results suggest that lutein biosynthetic pathway is the main one in the corona of Zhangzhou narcissus,the lutein biosynthetic pathway is replaced by β-carotene pathway and β-carotene accumulated in the red corona of ’Pink charm’ with the co-action of PDS,ZDS,LYCE and BCH genes.One possible explanation for the white of perianth in Zhangzhou narcissus is the difference in the structure and number of plastid.The results of this study will yield insights into the carotenoid biosynthesis in Chinese narcissus.