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| 嗜热脂肪土芽孢杆菌CHB1的5 L发酵罐发酵条件初探 | |
| 引用本文： | 张 慧,李活孙,邱宏端,林新坚.嗜热脂肪土芽孢杆菌CHB1的5 L发酵罐发酵条件初探[J].热带作物学报,2012,33(3):408-411. |
| 作者姓名： | 张 慧 李活孙 邱宏端 林新坚 |
| 作者单位： | 1. 福建省农业科学院土壤肥料研究所,福建福州,350013 2. 福建省农业科学院土壤肥料研究所,福建福州350013;福州大学生物科学与工程学院,福建福州 350002 3. 福州大学生物科学与工程学院,福建福州,350002 |
| 基金项目： | 福建省自然科学基金项目（No. 2009J01096）；福建省属公益类科研基本科研经费（闽财教[2009]0584号）。 |
| 摘 要： | 优化嗜热脂肪芽孢杆菌CHB1的5 L发酵罐发酵条件。通过单因素法优化发酵罐的通气量、转速、温度、pH等参数，并测定CHB1在5 L发酵罐中的生长曲线。结果表明，最佳发酵条件为：转速180 r/min、通气量6 L/min、发酵温度58 ℃、接种量4％，发酵过程自动流加乙酸控制pH值为8.0，培养21 h。采用自动流加乙酸控制pH值的方法，效果显著，控制pH值为8.0时，发酵效果最好，细胞生物量高达6.07×108 cfu/mL，约是不控制pH值发酵的对照组（3.5×108 cfu/mL）的2倍。 |
| 关 键 词： | 嗜热脂肪土芽孢杆菌 发酵罐 优化 |
Optimization of the Fermentation Conditions of 5L Fermenter for Geobacillus stearothermophilus CHB1 |
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| ZHANG Hui,LI Huosun,QIU Hongduan and LIN Xinjian.Optimization of the Fermentation Conditions of 5L Fermenter for Geobacillus stearothermophilus CHB1[J].Chinese Journal of Tropical Crops,2012,33(3):408-411. | |
| Authors: | ZHANG Hui LI Huosun QIU Hongduan and LIN Xinjian |
| Institution: | The Soil and Fertilizer Institute,Fujian Academy of Agricultural Sciences, Fuzhou;1 The Soil and Fertilizer Institute,Fujian Academy of Agricultural Sciences 2 College of Biological Science and Technology, Fuzhou University;College of Biological Science and Technology, Fuzhou University;The Soil and Fertilizer Institute,Fujian Academy of Agricultural Sciences |
| Abstract: | A single-factor method was used to optimize the conditions of Geobacillus stearothermophilus CHB1 such as ventilation volume,speed,temperature,pH and other parameters,and to determine the growth curve of CHB1 in a 5 L fermenter.The best fermentation conditions were: ventilation 6 L/min,speed 180r/min,inoculum 4% and culture temperature 58 ℃.The maximum cell biomass could be achieved by fermentation 21 h.Through the method of auto-fed acetic acid to control the pH of fermentation process,it could achieve a high-density fermentation of CHB1.The cell biology was as high as 6.07×108 cfu/ml.Using fed acid pH control pH8.0,it could achieve the maximum cell biomass up to 6.07×108 cfu/ml. |
| Keywords: | Geobacillus stearothermophilus Fermenter Optimization |
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