玫烟色棒束孢几丁质酶基因上游调控序列克隆与结构预测分析

为探明玫烟色棒束孢几丁质酶基因Ifuchi1的表达调控机理,根据Ifuchi1基因组全长核苷酸序列,采用基因组步移方法,获得Ifuchi1的5′-上游区序列(总长为2 402 bp)。与cDNA 序列进行比对,其中含有2个内含子。TFSEARCH 1.3 软件分析转录因子结合位点的结果显示,该序列中没有明显的TATA和CAAT框,含有4个可能的碳调控因子的结合位点5＇>(g/c)YggRg<3＇和3个氮调控因子的结合位点-相隔很近的GATA。其中含有可能的葡萄糖抑制调控序列和压力反应元件。本研究结果为深入研究玫烟色棒束孢几丁质酶基因的表达调控机制及其分子机理奠定了一定基础。

Cloning and Sequence Analysis of the Chitinase Gene Upstream from Isaria fumosorosea

The sequence of 5′-upstream region of the chitinase gene Ifuchi1 from Isaria fumosorosea was obtained by genome walking method according to the full-length genome nucleotide sequence of the Ifuchi1 to investigate the regulatory mechanism of Ifuchi1 gene expression.The 5′-upstream fragment of 2 402 bp of Ifuchi1 was successfully cloned and sequenced,and there are two introns compared to the cDNA of Ifuchi1 gene.Transcriptional elements in the 5′-upstream region of Ifuchi1 A were predicted by the TFSEARCH 1.3 software.The results showed that there are no obvious TATA and/or CAAT box,while there are four potential binding sites for carbon regulatory elements as 5’>(g/c)YggRg<3’and three potential binding sites for nitrogen as near GATA.There might be potential glucose inhibitor sequence and stress reaction elements among those elements.All of those results will be the basis for further clarifying the regulatory mechanism of chitinase Ifuchi1 expression.