| 奶牛布鲁氏菌的PCR鉴定 |
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| 引用本文： | 伊日盖,申之义,李平安,范伟兴,赵规,张七斤. 奶牛布鲁氏菌的PCR鉴定[J]. 畜牧与饲料科学, 2009, 30(3) |
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| 作者姓名： | 伊日盖 申之义 李平安 范伟兴 赵规 张七斤 |
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| 作者单位： | 1. 内蒙古农业大学,内蒙古,呼和浩特,010018
2. 中国动物卫生与流行病学中心,山东,青岛,266032 |
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| 摘 要： | 采集内蒙古某地区的可疑感染布鲁氏菌病奶牛的乳样及奶牛流产胎儿的肺、肝、脾等相关病料,分离并培养出11株病原菌,经BCSP31-PCR、VirB8-PCR、多重PCR及AMOS-PCR4种PCR方法鉴定,证实11株病原菌均为布鲁氏菌,并将11株菌定型为牛种5,6,9或3b型.
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| 关 键 词： | 奶牛 布鲁氏菌 分离 鉴定 |
Identification of Brucella in Dairy Cow by PCR |
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| Yirigai,SHEN Zhi-yi,LI Pin-gan,FAN Wei-xing,ZHAO Kun,ZHANG Qi-jin. Identification of Brucella in Dairy Cow by PCR[J]. Animal Husbandry and Feed Science, 2009, 30(3) |
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| Authors: | Yirigai SHEN Zhi-yi LI Pin-gan FAN Wei-xing ZHAO Kun ZHANG Qi-jin |
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| Affiliation: | 1.Inner Mongolia Agricultural University;Hohhot 010018;China;2.China Animal Health and Epidemiology Center;Qingdao 266032;China |
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| Abstract: | The milk samples and relative samples of lung,liver,spleen etc.in aborted fetus of dairy cow infected with Brucella suspiciously in an area of Inner Mongolia were collected and 11 pathogenic strains were isolate and cultured.11 pathogenic strains were identified as Brucella and confirmed as B.abortus biovar 5,6,9 or 3b by using 4 kinds of PCR methods as BCSP31-PCR,VirB8-PCR,multiplex PCR and AMOS-PCR. |
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| Keywords: | PCR |
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