超高效液相色谱-串联质谱法测定植物源调味料中氯虫苯甲酰胺残留

本研究建立了超高效液相色谱-串联质谱（UPLC-MS/MS）测定植物源调味料中氯虫苯甲酰胺残留量的分析方法。调味料样品经改进后的QuEChERS方法前处理,选择C₁₈超高效液相色谱柱进行分离,采用电喷雾离子源正离子模式、多反应监测方式进行采集,外标法定量。结果表明,方法在0.005~0.200 μg/mL范围内线性相关系数均优于0.999,加标回收率在70%~108%之间,相对标准偏差在1.9%~10%之间。方法检出限为0.002 mg/kg,定量限为0.005 mg/kg。该方法操作简便快速、灵敏、准确性高,适用于植物源调味料中氯虫苯甲酰胺的残留量检测。

Determination of Chlorantraniliprole Residues in Plant-derived Condiment by UPLC-MS/MS

A simple and efficient method based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) detection was developed to analyze the residue of chlorantraniliprole in plant-derived condiment. The condiment samples were pretreated with a modified QuEChERS method, and separated on an UPLC C₁₈ column. The analyses of chlorantraniliprole were operated with electrospray ionization mass spectrometry under positive mode using a multiple reaction monitoring mode, and then quantitatived by an external standard method. The results showed that the correlation coefficients of the calibration curves were over 0.999 in linear range of 0.005-0.200 μg/mL. The average recoveries at three spiked concentration levels varied from 70% to 108% with relative standard deviations (RSDs) of 1.9%-10%. The limits of detection (LOD) and quantitation (LOQ) was 0.002 mg/kg and 0.005 mg/kg, respectively. In conclusion, this simple and efficient method meet the method validation requirements for the determination of chlorantraniliprole residues in plant-derived condiment.