玉米RNA对小鼠脂肪沉积的影响

本试验旨在探讨玉米RNA对小鼠脂肪沉积的影响。将20只28日龄C57BL/6J小鼠随机分为2组,每组10个重复,每个重复1只小鼠。对照组灌服生理盐水,试验组灌服玉米RNA(100μg/d),试验期4周。试验结束时检测小鼠的生长性能、血清生化指标、体组成、体成像、器官指数及脂肪相关基因的表达。结果显示:试验组小鼠体重和附睾脂肪组织指数显著低于对照组(P<0.05),且整体脂肪含量明显减少,表明小鼠脂肪沉积减少。血脂检测结果显示,与对照组相比,试验组血清球蛋白(GLB)含量显著增加(P<0.05),高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)和总胆固醇(TC)含量极显著增加(P<0.01);脂肪相关基因检测结果显示,与对照组相比,试验组脂肪合成相关基因脂肪酸合成酶(FAS)和CCAAT增强子结合蛋白α(C/EBP-α)mRNA相对表达量极显著上调(P<0.01),过氧化物酶体增殖剂激活受体-γ(PPAR-γ)及脂肪分解基因脂肪甘油三酯脂肪酶(ATGL)和激素敏感脂酶(HSL)mRNA相对表达量显著上调(P<0.05),表明小鼠脂肪合成和脂肪分解增加。综上所述,玉米RNA能同时促进小鼠脂肪合成代谢和脂肪分解代谢,最终抑制脂肪沉积,为植物源核酸调控动物脂肪沉积影响因素的探索提供新证据。

Effects of Maize RNA on Fat Deposition of Mice

The experiment was aimed to investigate the effects of maize RNA on fat deposition of mice.Twenty 28-day-old C57BL/6J mice were randomly divided into two groups.There were 10 replicates in each group,with one mouse per replicate.Normal saline(control group)and total RNA of maize(experiment group,100μg/d)were given by intragastric administration for 4 weeks.At the end of the experiment,growth performance,serum biochemical indexes,organ indexes,body imaging,body composition,and the relative levels of fat-related genes of the mice were detected.The results showed that the body weight,adipose tissue ratio and epididymal adipose index of the mice were reduced in the experimental group.Serum globulin(GLB),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)and total cholesterol(TC)contents were significantly increased in the experimental group compared with the mice in the control group.Both adipogenesis genes(fatty acid synthetase and CCAAT enhancer binding protein alpha)and lipolytic genes(adipose triglyceride lipase and hormone sensitive lipase)relative mRNA expression levels in epididymal adipose tissue were significantly increased by maize RNA administration.In summary,maize RNA can promote the occurrence of fat metabolism and inhibit fat deposition.This study provides new evidences for plant-derived nucleic acids regulating fat metabolism in animals.