| 慈竹木质素合成酶基因4CL RNAi载体构建与烟草转化 |
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| 引用本文： | 周建英,曹颖,孙霞,陈珂,卢学琴,胡尚连. 慈竹木质素合成酶基因4CL RNAi载体构建与烟草转化[J]. 福建林业科技, 2010, 37(2). DOI: 10.3969/j.issn.1002-7351.2010.02.007 |
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| 作者姓名： | 周建英 曹颖 孙霞 陈珂 卢学琴 胡尚连 |
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| 作者单位： | 1. 西南科技大学生命科学与工程学院,四川,绵阳,621010
2. 西南科技大学生命科学与工程学院,四川,绵阳,621010;中国科学院东北地理与农业生态研究所,黑龙江,哈尔滨,150081 |
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| 基金项目： | 四川省应用基础研究基金资助项目 |
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| 摘 要： | 根据慈竹4-香豆酸辅酶A连接酶(Na4CL)全长cDNA序列,通过RT-PCR从慈竹幼笋cDNA中扩增约600 bp的保守片段。DNAMAN多重比对分析表明,该目标片段与3条烟草4CL基因同源性达84.08%。将目标片段插入到pSK-int中间表达载体中,获得pSK-4CL-RNAi中间表达载体,然后将其转入植物双元表达载体pBI121中,构建该基因的shRNA表达载体,并利用农杆菌介导法转化烟草(K326)。对获得的抗性再生植株的PCR检测分析初步表明该基因已插入烟草基因组中。
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| 关 键 词： | 慈竹 4CL RNAi 烟草 |
Construction of RNAi Carrier of 4CL Gene of Neosinocalamus affinis and Its Transformation into Tobacco |
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| ZHOU Jian-ying,CAO Ying,SUN Xia,CHEN Ke,LU Xue-qin,HU Shang-lian. Construction of RNAi Carrier of 4CL Gene of Neosinocalamus affinis and Its Transformation into Tobacco[J]. Journal of Fujian Forestry Science and Technology, 2010, 37(2). DOI: 10.3969/j.issn.1002-7351.2010.02.007 |
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| Authors: | ZHOU Jian-ying CAO Ying SUN Xia CHEN Ke LU Xue-qin HU Shang-lian |
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| Affiliation: | ZHOU Jian-ying1,CAO Ying1,SUN Xia1,2,CHEN Ke1,LU Xue-qin1,HU Shang-lian1(1.Life Science and Engineering College,Southwest University of Science and Technology,Mianyang 621010,Sichuan,China,2.Northeast Institute of Geography and Agroecology,Chinese Academy of Science,Harbin150081,Heilongjiang,China) |
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| Abstract: | The conserved fragments with about 600 bp were amplified by RT-PCR by using cDNA as a template from the young shoot of Neosinocalamus affinis according to full-length sequence of 4CL of Neosinocalamus affinis(Na4CL) in this study.DNAMAN multiple comparison analysis results showed that the 600 bp conserved-fragment of Na4CL was the most similar to 4CL genes of tobacco up to 84.08% by DNAMAN.The PSK-int expression vector was constructed though inserting the 600bp conserved-fragment of Na4CL in Neosinocalamus ... |
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| Keywords: | 4CL RNAi |
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