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甜瓜雄全同株和雌雄异花同株的RAPD标记
引用本文:张桂芬,李计红,张建农.甜瓜雄全同株和雌雄异花同株的RAPD标记[J].甘肃农业大学学报,2011,46(3):35-37,42.
作者姓名:张桂芬  李计红  张建农
作者单位:1. 甘肃农业职业技术学院,甘肃兰州,730020
2. 兰州大学生命科学学院,甘肃兰州,730000
3. 甘肃农业大学农学院,甘肃兰州,730070
摘    要:以甜瓜雄全同株和雌雄异花同株近等基因系为试验材料,选用300条随机引物进行性型性状的RAPD标记.结果表明:S152号引物经多次重复均能扩增出清晰、稳定且有差异的DNA条带,在雄全同株的植株中能扩增出一条分子量约为550 bp的特异条带,标为S152550.在F2代分离群体中,根据特异标记在田间实际雄全同株与雌雄异花同...

关 键 词:甜瓜  性型  近等基因系  RAPD标记

The RAPD marker for melon andromonoecy and monoecy
ZHANG Gui-fen,LI Ji-hong,ZHANG Jian-nong.The RAPD marker for melon andromonoecy and monoecy[J].Journal of Gansu Agricultural University,2011,46(3):35-37,42.
Authors:ZHANG Gui-fen  LI Ji-hong  ZHANG Jian-nong
Abstract:In order to explore the DNA molecular marker of melon sexual character,near-isogenic lines of andromonoecy and monoecy were used as materials to select RAPD marker with 300 random primers.The results showed that a 550 bp band was amplified from andromonoecy by S152 primer,which makerd as S152550.The recombination rate between the marker and the sexual character was 11% according to the results of the marker was in andromonoecy and monoecy or not.
Keywords:melon  sexual type  near-isogenic lines  RAPD marker
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