| PttGA20-氧化酶基因dsRNA抑止载体的构建(英文) |
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| 引用本文： | 周冰彬,陈晓阳. PttGA20-氧化酶基因dsRNA抑止载体的构建(英文)[J]. 农业科学与技术, 2008, 9(4): 56-59 |
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| 作者姓名： | 周冰彬 陈晓阳 |
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| 作者单位： | [1]北京林业大学林木花卉遗传育种教育部重点实验室; [2]华南农业大学林学院 |
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| 摘 要： | [Objective] Genetic Engineering technology was used to regulate the expression of PttGA20-oxidase gene thus restrained plant height growth and internode elongation for cultivating dwarfed plant.[Method] Based on the RNAi principle,the gene specific sequences of PttGA20-oxidase in the antisense and sense orientations interrupted by a gene sequence from GUS were cloned into a binary vector pBI121.The selection marker gene npt Ⅱ was replaced with bar gene to RNAi plasmid.[Result] After undergone different endonuclease restrictions,the constructed constraint vector released different segments whose sizes were similar to that of target segment,which demonstrated that the RNAi plasmid of PttGA20-oxidase gene was successfully constructed.[Conclusion] The experiment provided a new way for culturing dwarfed plant.
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| 关 键 词： | Gibberellins PttGA 20-oxidase gene RNAi Vector construction |
RNAi Plasmid Construction of PttGA 20-oxidase Gene |
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| ZHOU Bing-bin,CHEN Xiao-yang. RNAi Plasmid Construction of PttGA 20-oxidase Gene[J]. Agricultural Science ＆ Technology, 2008, 9(4): 56-59 |
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| Authors: | ZHOU Bing-bin CHEN Xiao-yang |
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| Affiliation: | ZHOU Bing-bin1,CHEN Xiao-yang2 1.Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing 100083;2.College of forestry,South China Agricultural University,Guangzhou 510642 |
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| Abstract: | [Objective] Genetic Engineering technology was used to regulate the expression of PttGA20-oxidase gene thus restrained plant height growth and internode elongation for cultivating dwarfed plant. [Method] Based on the RNAi principle, the gene specific sequences of PttGA20-oxidase in the antisense and sense orientations interrupted by a gene sequence from GUS were cloned into a binary vector pBI121. The selection marker gene npt Ⅱ was replaced with bar gene to RNAi plasmid. [Result] After undergone different endonuclease restrictions, the constructed constraint vector released different segments whose sizes were similar to that of target segment, which demonstrated that the RNAi plasmid of PttGA20-oxidase gene was successfully constructed. [Conclusion] The experiment provided a new way for culturing dwarfed plant. |
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| Keywords: | Gibberellins PttGA 20-oxidase gene RNAi Vector construction |
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